Theoretical and Applied Genetics

, 117:157

Genetic analysis of opaque2 modifier loci in quality protein maize

  • David R. Holding
  • Brenda G. Hunter
  • Taijoon Chung
  • Bryan C. Gibbon
  • Clark F. Ford
  • Arvind K. Bharti
  • Joachim Messing
  • Bruce R. Hamaker
  • Brian A. Larkins
Original Paper

DOI: 10.1007/s00122-008-0762-y

Cite this article as:
Holding, D.R., Hunter, B.G., Chung, T. et al. Theor Appl Genet (2008) 117: 157. doi:10.1007/s00122-008-0762-y

Abstract

Quality protein maize (QPM) was created by selecting genetic modifiers that convert the starchy endosperm of an opaque2 (o2) mutant to a hard, vitreous phenotype. Genetic analysis has shown that there are multiple, unlinked o2 modifiers (Opm), but their identity and mode of action are unknown. Using two independently developed QPM lines, we mapped several major Opm QTLs to chromosomes 1, 7 and 9. A microarray hybridization performed with RNA obtained from true breeding o2 progeny with vitreous and opaque kernel phenotypes identified a small group of differentially expressed genes, some of which map at or near the Opm QTLs. Several of the genes are associated with ethylene and ABA signaling and suggest a potential linkage of o2 endosperm modification with programmed cell death.

Supplementary material

122_2008_762_MOESM1_ESM.xls (28 kb)
Supplementary Table 1 (XLS 28 kb)
122_2008_762_MOESM2_ESM.xls (18 kb)
Supplementary Table 2 (XLS 17 kb)

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • David R. Holding
    • 1
  • Brenda G. Hunter
    • 1
  • Taijoon Chung
    • 1
    • 2
  • Bryan C. Gibbon
    • 1
    • 3
  • Clark F. Ford
    • 4
  • Arvind K. Bharti
    • 5
  • Joachim Messing
    • 5
  • Bruce R. Hamaker
    • 6
  • Brian A. Larkins
    • 1
  1. 1.Department of Plant SciencesUniversity of ArizonaTucsonUSA
  2. 2.Department of GeneticsUniversity of Wisconsin-MadisonMadisonUSA
  3. 3.Department of BiologyBaylor UniversityWacoUSA
  4. 4.Department of Food Science and Human NutritionIowa State UniversityAmesUSA
  5. 5.Waksman Institute of MicrobiologyRutgers UniversityPiscatawayUSA
  6. 6.Department of Food SciencePurdue UniversityWest LafayetteUSA