Theoretical and Applied Genetics

, Volume 114, Issue 5, pp 803–814

Generation and characterization of low phytic acid germplasm in rice (Oryza sativa L.)

  • Qing-Long Liu
  • Xiu-Hong Xu
  • Xue-Liang Ren
  • Hao-Wei Fu
  • Dian-Xing Wu
  • Qing-Yao Shu
Original Paper

DOI: 10.1007/s00122-006-0478-9

Cite this article as:
Liu, Q., Xu, X., Ren, X. et al. Theor Appl Genet (2007) 114: 803. doi:10.1007/s00122-006-0478-9

Abstract

Phytic acid (PA, myo-inositol 1,2,3,4,5,6-hexakisphosphate), or its salt form, phytate, is commonly regarded as the major anti-nutritional component in cereal and legume grains. Breeding of low phytic acid (lpa) crops has recently been considered as a potential way to increase nutritional quality of crop products. In this study, eight independent lpa rice mutant lines from both indica and japonica subspecies were developed through physical and chemical mutagenesis. Among them, five are non-lethal while the other three are homozygous lethal. None of the lethal lines could produce homozygous lpa plants through seed germination and growth under field conditions, but two of them could be rescued through in vitro culture of mature embryos. The non-lethal lpa mutants had lower PA content ranging from 34 to 64% that of their corresponding parent and four of them had an unchanged total P level. All the lpa mutations were inherited in a single recessive gene model and at least four lpa mutations were identified mutually non-allelic, while the other two remain to be verified. One mutation was mapped on chromosome 2 between microsatellite locus RM3542 and RM482, falling in the same region as the previously mapped lpa1-1 locus did; another lpa mutation was mapped on chromosome 3, tightly linked to RM3199 with a genetic distance of 1.198 cM. The latter mutation was very likely to have happened to the LOC_Os03g52760, a homolog of the maize myo-inositol kinase (EC 2.7.1.64) gene. The present work greatly expands the number of loci that could influence the biosynthesis of PA in rice, making rice an excellent model system for research in this area.

Keywords

Oryza sativa L. Low phytic acid (lpa)MutationGene mappingMyo-inositol kinase

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Qing-Long Liu
    • 1
    • 2
  • Xiu-Hong Xu
    • 1
  • Xue-Liang Ren
    • 1
  • Hao-Wei Fu
    • 1
    • 3
  • Dian-Xing Wu
    • 1
  • Qing-Yao Shu
    • 1
    • 4
  1. 1.IAEA-Zhejiang University Collaborating Center, Institute of Nuclear Agricultural SciencesZhejiang UniversityHangzhouChina
  2. 2.Institute of Crop Science and Nuclear Technology UtilizationZhejiang Academy of Agricultural SciencesHangzhouChina
  3. 3.Jiaxing Academy of Agricultural SciencesJiaxingChina
  4. 4.Joint FAO/IAEA Division of Nuclear Techniques in Food and AgricultureInternational Atomic Energy AgencyViennaAustria