Theoretical and Applied Genetics

, Volume 114, Issue 1, pp 21–30

Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat

  • E. S. Lagudah
  • H. McFadden
  • R. P. Singh
  • J. Huerta-Espino
  • H. S. Bariana
  • W. Spielmeyer
Original Paper

DOI: 10.1007/s00122-006-0406-z

Cite this article as:
Lagudah, E.S., McFadden, H., Singh, R.P. et al. Theor Appl Genet (2006) 114: 21. doi:10.1007/s00122-006-0406-z

Abstract

Wheat expressed sequence tags (wESTs) were identified in a genomic interval predicted to span the Lr34/Yr18 slow rusting region on chromosome 7DS and that corresponded to genes located in the syntenic region of rice chromosome 6 (between 2.02 and 2.38 Mb). A subset of the wESTs was also used to identify corresponding bacterial artificial chromosome (BAC) clones from the diploid D genome of wheat (Aegilops tauschii). Conservation and deviation of micro-colinearity within blocks of genes were found in the D genome BACs relative to the orthologous sequences in rice. Extensive RFLP analysis using the wEST derived clones as probes on a panel of wheat genetic stocks with or without Lr34/Yr18 revealed monomorphic patterns as the norm in this region of the wheat genome. A similar pattern was observed with single nucleotide polymorphism analysis on a subset of the wEST derived clones and subclones from corresponding D genome BACs. One exception was a wEST derived clone that produced a consistent RFLP pattern that distinguished the Lr34/Yr18 genetic stocks and well-established cultivars known either to possess or lack Lr34/Yr18. Conversion of the RFLP to a codominant sequence tagged site (csLV34) revealed a bi-allelic locus, where a variant size of 79 bp insertion in an intron sequence was associated with lines or cultivars that lacked Lr34/Yr18. This association with Lr34/Yr18 was validated in wheat cultivars from diverse backgrounds. Genetic linkage between csLV34 and Lr34/Yr18 was estimated at 0.4 cM

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • E. S. Lagudah
    • 1
  • H. McFadden
    • 1
  • R. P. Singh
    • 2
  • J. Huerta-Espino
    • 3
  • H. S. Bariana
    • 4
  • W. Spielmeyer
    • 1
  1. 1.CSIRO Plant IndustryCanberraAustralia
  2. 2.International Maize and Wheat Improvement Center (CIMMYT)Mexico, D.F.Mexico
  3. 3.Campo Experimental Valle de Mexico-INIFAPChapingo, Edo. de MexicoMexico
  4. 4.Plant Breeding InstituteUniversity of SydneyCobbittyAustralia

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