Theoretical and Applied Genetics

, Volume 112, Issue 7, pp 1349–1359

An enhanced microsatellite map of diploid Fragaria

  • D. J. Sargent
  • J. Clarke
  • D. W. Simpson
  • K. R. Tobutt
  • P. Arús
  • A. Monfort
  • S. Vilanova
  • B. Denoyes-Rothan
  • M. Rousseau
  • K. M. Folta
  • N. V. Bassil
  • N. H. Battey
Original Paper

DOI: 10.1007/s00122-006-0237-y

Cite this article as:
Sargent, D., Clarke, J., Simpson, D. et al. Theor Appl Genet (2006) 112: 1349. doi:10.1007/s00122-006-0237-y
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Abstract

A total of 45 microsatellites (SSRs) were developed for mapping in Fragaria. They included 31 newly isolated codominant genomic SSRs from F. nubicola and a further 14 SSRs, derived from an expressed sequence tagged library (EST-SSRs) of the cultivated strawberry, F. × ananassa. These, and an additional 64 previously characterised but unmapped SSRs and EST-SSRs, were scored in the diploid Fragaria interspecific F2 mapping population (FV×FN) derived from a cross between F. vesca 815 and F. nubicola 601. The cosegregation data of these 109 SSRs, and of 73 previously mapped molecular markers, were used to elaborate an enhanced linkage map. The map is composed of 182 molecular markers (175 microsatellites, six gene specific markers and one sequence-characterised amplified region) and spans 424 cM over seven linkage groups. The average marker spacing is 2.3 cM/marker and the map now contains just eight gaps longer than 10 cM. The transferability of the new SSR markers to the cultivated strawberry was demonstrated using eight cultivars. Because of the transferable nature of these markers, the map produced will provide a useful reference framework for the development of linkage maps of the cultivated strawberry and for the development of other key resources for Fragaria such as a physical map. In addition, the map now provides a framework upon which to place transferable markers, such as genes of known function, for comparative mapping purposes within Rosaceae.

Keywords

FragariaGenetic mappingMicrosatellitesESTFunctional genomics

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • D. J. Sargent
    • 1
    • 6
  • J. Clarke
    • 1
  • D. W. Simpson
    • 1
  • K. R. Tobutt
    • 1
  • P. Arús
    • 2
  • A. Monfort
    • 2
  • S. Vilanova
    • 2
  • B. Denoyes-Rothan
    • 3
  • M. Rousseau
    • 3
  • K. M. Folta
    • 4
  • N. V. Bassil
    • 5
  • N. H. Battey
    • 6
  1. 1.East Malling Research (EMR)KentUK
  2. 2.Departament de Genética VegetalInstitut de Recerca i Tecnologia Agroalimentáries (IRTA)Cabrils (Barcelona)Spain
  3. 3.INRA-Unité de Recherche sur les Espèces Fruitières et la VigneVillenave d’Ornon CedexFrance
  4. 4.Plant Molecular and Cellular Biology Program and Horticultural Sciences DepartmentThe University of FloridaGainesvilleUSA
  5. 5.USDA-ARS NCGRCorvallisUSA
  6. 6.School of Plant SciencesThe University of ReadingReadingUK