Theoretical and Applied Genetics

, Volume 110, Issue 8, pp 1499–1504

Coding region single nucleotide polymorphism in the barley low-pI, α-amylase gene Amy32b

  • K. Machova Polakova
  • L. Kucera
  • D. A. Laurie
  • K. Vaculova
  • J. Ovesna
Original Paper

DOI: 10.1007/s00122-005-1985-9

Cite this article as:
Polakova, K.M., Kucera, L., Laurie, D.A. et al. Theor Appl Genet (2005) 110: 1499. doi:10.1007/s00122-005-1985-9

Abstract

Barley α-amylase variability influences the quality of barley grain in the brewing, feed and food industries. α-Amylase proteins are encoded by multigene families in cereals, and this study focused on the barley Amy32b gene. We identified coding region single nucleotide polymorphism (cSNP) and insertion/deletion variation in DNA sequences, which resulted in amino acid substitution and stop codon formation, respectively. The substitution affected the β1 strand in domain C, whereas the stop codon removed the β5 strand. Possible effects of these changes on the protein are discussed. A cSNP in the coding region of the Amy32b gene was used as a specific marker to map Amy32b loci on chromosome 7H.

Keywords

α-Amylase Amy32b Barley Coding region single nucleotide polymorphism Linkage map Protein sequence 

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • K. Machova Polakova
    • 1
  • L. Kucera
    • 1
  • D. A. Laurie
    • 2
  • K. Vaculova
    • 3
  • J. Ovesna
    • 1
  1. 1.Research Institute of Crop ProductionPrague-RuzyneCzech Republic
  2. 2.John Innes CentreNorwichUK
  3. 3.Agriculture Research Institute, KromerizKromerizCzech Republic

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