Theoretical and Applied Genetics

, Volume 110, Issue 8, pp 1429–1438

Root response to Fusarium solani f. sp . glycines: temporal accumulation of transcripts in partially resistant and susceptible soybean

  • M. J. Iqbal
  • Satsuki Yaegashi
  • Rubina Ahsan
  • Kay L. Shopinski
  • David A. Lightfoot
Original Paper

DOI: 10.1007/s00122-005-1969-9

Cite this article as:
Iqbal, M.J., Yaegashi, S., Ahsan, R. et al. Theor Appl Genet (2005) 110: 1429. doi:10.1007/s00122-005-1969-9

Abstract

Sudden death syndrome (SDS) of soybean is a complex of root rot disease caused by the semi-biotrophic fungus Fusarium solani f. sp. glycines (Fsg) and a leaf scorch disease caused by toxins produced by the pathogen in the roots. Development of partial rate-reducing resistance in roots to SDS was studied. The recombinant inbred line 23 (RIL23) that carried resistance conferred by six quantitative trait loci (QTL) derived from cultivars ‘Essex’ × ‘Forrest’ was compared to the susceptible cultivar Essex. Roots of RIL23 and its susceptible parent Essex were inoculated with Fsg. Transcript abundance (TA) of 191 ESTs was studied at five time points after inoculation. For most of the genes, there was an initial decrease in TA in the inoculated roots of both genotypes. By days 7 and 10 the inoculated roots of Essex failed to increase expression of the transcripts of defense-related genes. In RIL23 inoculated roots, the TA of 81 genes was increased by at least two-fold at day 3 (P=0.004), 88 genes at day 7 (P=0.0023) and 129 genes at day 10 (P=0.0026). A set of 35 genes maintained at least a two-fold higher abundance at all three time points. The increase in TA in RIL23 was in contrast to that observed in Essex where most of the ESTs showed either no change or a decreased TA. The ESTs with an increased TA had homology to the genes involved in resistance (analogs), signal transduction, plant defense, cell wall synthesis and transport of metabolites. Pathways that responded included the protein phosphorylation cascade, the phospholipase cascade and the phenolic natural products pathways, including isoflavone and cell wall synthesis.

Keywords

Sudden death syndrome of soybean Transcript abundance Defense related genes Temporal accumulation 

Supplementary material

122_2005_1969_ESM_supp.pdf (348 kb)
(PDF 348 KB)

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • M. J. Iqbal
    • 1
  • Satsuki Yaegashi
    • 1
  • Rubina Ahsan
    • 1
  • Kay L. Shopinski
    • 1
  • David A. Lightfoot
    • 1
  1. 1.Plant Biotechnology and Genome Center, Department of Plant, Soil and Agricultural SystemsSouthern Illinois UniversityCarbondaleUSA

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