Theoretical and Applied Genetics

, Volume 110, Issue 7, pp 1210–1217

Cross-species amplification of Medicago truncatula microsatellites across three major pulse crops

  • M. V. Gutierrez
  • M. C. Vaz Patto
  • T. Huguet
  • J. I. Cubero
  • M. T. Moreno
  • A. M. Torres
Original Paper

DOI: 10.1007/s00122-005-1951-6

Cite this article as:
Gutierrez, M.V., Vaz Patto, M.C., Huguet, T. et al. Theor Appl Genet (2005) 110: 1210. doi:10.1007/s00122-005-1951-6
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Abstract

Model plants are facilitating the genetic characterization and comparative mapping of a number of traditional crops. Medicago truncatula has been widely accepted as a model plant to this end as it provides the essential tools for multiple aspects of legume genetics and genomics. A large set of markers from highly conserved M. truncatula gene regions is being created and used to establish a worldwide framework for comparative genomic studies in legumes. We have investigated the potential for cross-species amplification of 209 expressed sequence tag (EST)-based and 33 bacterial artificial chromosome (BAC)-based microsatellites from M. truncatula in the three most important European legume pulses—pea, faba bean and chickpea—that might facilitate future comparative mapping. Our results revealed significant transferability of M. truncatula microsatellites to the three pulses (40% in faba bean, 36.3% in chickpea and 37.6% in pea). The percentage of M. truncatula EST-SSRs (simple sequence repeats) amplified in the three crops (39–43%) was twofold higher than that of the genomic SSRs (21–24%). Sequence analysis determined that the level of conservation in the microsatellite motif was very low, while the flanking regions were generally well conserved. The variations in the sequences were mainly due to changes in the number of repeat motifs in the microsatellite region combined with indel and base substitutions. None of the functional microsatellites showed direct polymorphism among the parental genotypes tested, consequently preventing their immediate use for mapping purposes.

Supplementary material

S1 List of Medicago truncatula EST and BAC microsatellite primers used in the present study

122_2005_1951_ESM_Table_1.pdf (240 kb)
(PDF 240 KB)

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • M. V. Gutierrez
    • 1
  • M. C. Vaz Patto
    • 2
  • T. Huguet
    • 3
  • J. I. Cubero
    • 4
  • M. T. Moreno
    • 1
  • A. M. Torres
    • 1
  1. 1.CIFA-Alameda del Obispo, IFAPAArea de Mejora y BiotecnologíaCórdobaSpain
  2. 2.Plant Cell Biotechnology LaboratoryInstituto de Tecnología Química e Biológica (ITQB)OeirasPortugal
  3. 3.Laboratoire des Interactions Plantes Micro-organismes (LIPM)UMR CNRS/INRACastanet Tolosan CedexFrance
  4. 4.Departamento de Genética, E.T.S.I.A.M.University of CórdobaCórdobaSpain

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