Theoretical and Applied Genetics

, Volume 110, Issue 6, pp 1099–1107

Allelic variation at the linked AP1 and PhyC loci in hexaploid wheat is associated but not perfectly correlated with vernalization response

Original Paper

DOI: 10.1007/s00122-005-1938-3

Cite this article as:
Beales, J., Laurie, D.A. & Devos, K.M. Theor Appl Genet (2005) 110: 1099. doi:10.1007/s00122-005-1938-3


Vernalization requirement is an important trait in temperate crop plants such as wheat and must be considered when selecting varieties for cultivation under different climatic conditions. To determine the growth habit of wheat varieties, plants need to be grown under different vernalization regimes, a lengthy but necessary process for breeders involved in crossing winter with spring germplasm. If haplotypes can be associated with growth habit, then molecular marker assays that are reliable, cheap, and quick can be developed to assist in the selection of plants with the desired phenotype. We have analyzed 81 accessions that have different vernalization requirements and putative different origins of spring habit for sequence variation at the Apetala1 (AP1) locus, which underlies Vrn-1, and at the linked Phytochrome C (PhyC) locus. Good correspondence was found between the AP1 genotype and the PhyC haplotype for 77 of the 81 accessions. Two varieties displayed a recombination event between the AP1 and PhyC loci, and one variety carried a recombinant PhyC gene. In addition, one variety carried an apparent AP1 winter allele, but displayed the Vrn-A1 spring habit. The PhyC haplotype for this variety also indicated the presence of a Vrn-A1 spring allele. Our data suggest that both the AP1 promoter region and PhyC SNPs can be used as diagnostic markers for vernalization response at the vrn-A1 locus, but that neither are perfect tags.


Apetala1Diagnostic markersHaplotypePhytochrome CVernalization

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  1. 1.John Innes CentreNorwichUK
  2. 2.Department of Crop and Soil Sciences and Department of Plant BiologyUniversity of GeorgiaAthensUSA