Original Paper

Theoretical and Applied Genetics

, Volume 112, Issue 4, pp 674-687

First online:

Characterization and mapping of R Pi-ber , a novel potato late blight resistance gene from Solanum berthaultii

  • G. M. RauscherAffiliated withDepartment of Plant Pathology, Cornell University
  • , C. D. SmartAffiliated withDepartment of Plant Pathology, Cornell UniversityDepartment of Plant Pathology, Cornell University
  • , I. SimkoAffiliated withDepartment of Horticulture, Cornell UniversityUSDA-ARS
  • , M. BonierbaleAffiliated withCentro Internacional de la Papa
  • , H. MaytonAffiliated withDepartment of Plant Pathology, Cornell University
  • , A. GreenlandAffiliated withSyngenta Jealott’s HillNational Institute for Agricultural Botany
  • , W. E. FryAffiliated withDepartment of Plant Pathology, Cornell University Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Phytophthora infestans, the causal agent of late blight, threatens potato production worldwide. An important tool in the management of the disease is the use of resistant varieties. Eleven major resistance genes have been identified and introgressed from Solanum demissum. However, new sources of resistance are continually sought. Here, we report the characterization and refined genetic localization of a resistance gene previously identified as Rber in a backcross progeny of Solanum tuberosum and Solanum berthaultii. In order to further characterize Rber, we developed a set of P. infestans isolates capable of identifying each of the 11 R-genes known to confer resistance to late blight in potato. Our results indicate that Rber is a new resistance gene, different from those recognized in S. demissum, and therefore, it has been named R Pi-ber according to the current system of nomenclature. In order to add new molecular markers around R Pi-ber , we used a PCR-based mapping technique, named MASP-map, which located R Pi-ber in a 3.9 cM interval between markers CT240 and TG63 on potato chromosome X. The location of R Pi-ber coincides with an area involved in resistance to different pathogens of potato and tomato.