Original Paper

Theoretical and Applied Genetics

, Volume 111, Issue 8, pp 1489-1494

First online:

A ‘Chinese Spring’ wheat (Triticum aestivum L.) bacterial artificial chromosome library and its use in the isolation of SSR markers for targeted genome regions

  • B. ShenAffiliated withCSIRO Plant Industry
  • , D.M. WangAffiliated withCSIRO Plant IndustryCollege of life science, Hebei Agricultural University
  • , C.L. McIntyreAffiliated withCSIRO Plant Industry
  • , C.J. LiuAffiliated withCSIRO Plant Industry Email author 

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Abstract

A bacterial artificial chromosome (BAC) library was constructed from the bread wheat (Triticum aestivum L.) genotype ‘Chinese Spring’ (‘CS’). The library consists of 395,136 clones with an estimated average insert size of 157 kb. This library provides an estimated 3.4-fold genome coverage for this hexaploid species. The genome coverage was confirmed by RFLP analysis of single-copy RFLP clones. The CS BAC library was used to develop simple sequence repeat (SSR) markers for targeted genome regions using five sequence-tagged-site (STS) markers designed from the chromosome arm of 3BS. The SSR markers for the targeted genome region were successfully obtained. However, similar numbers of new SSR markers were also generated for the other two homoeologous group 3 chromosomes. This data suggests that BAC clones belonging to all three chromosomes of homoeologous group 3 were isolated using the five STS primers. The potential impacts of these results on marker isolation in wheat and on library screening in general are discussed.