Original Paper

Theoretical and Applied Genetics

, Volume 111, Issue 7, pp 1322-1329

First online:

Rationalising germplasm collections: a case study for wheat

  • O. DobrovolskayaAffiliated withInstitut für Pflanzengenetik und Kulturpflanzenforschung (IPK)Siberian Branch of the Russian Academy of Sciences, Institute of Cytology and Genetics
  • , U. SalehAffiliated withInstitut für Pflanzengenetik und Kulturpflanzenforschung (IPK)Regional Agricultural Research Station
  • , L. Malysheva-OttoAffiliated withInstitut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
  • , M.S. RöderAffiliated withInstitut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
  • , A. BörnerAffiliated withInstitut für Pflanzengenetik und Kulturpflanzenforschung (IPK)

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Abstract

In total 70 genebank accessions comprising 50 hexaploid, 12 tetraploid and 8 diploid wheats of the Gatersleben collection were selected based on the screening of the passport data for identical cultivar names or accession numbers of the donor genebanks. Twelve potential duplicate groups consisting of three to nine accessions with identical names/numbers were selected and analysed with DNA markers (microsatellites). A bootstrap approach based on re-sampling of both microsatellite markers and alleles within marker loci was used to test for homogeneity. Although several homogeneous groups were identified it became clear that cultivar name identity alone did not allow the determination of duplicates. A combination of SSR-analysis followed by the bootstrap method and database survey considering the botanical classification and other data (origin, growth habit and donor) available is recommended in order to determine duplicates. A procedure for the identification of duplicates and their further handling in ex situ genebanks is discussed.

Keywords

Duplicates Ex situ genebank collections Microsatellites Wheat Triticum spp.