Theoretical and Applied Genetics

, Volume 110, Issue 6, pp 995–1002

Structural and distributional variation of mitochondrial rps2 genes in the tribe Triticeae (Poaceae)

  • N. Kubo
  • B. Salomon
  • T. Komatsuda
  • R. von Bothmer
  • K. Kadowaki
Original Paper

DOI: 10.1007/s00122-004-1839-x

Cite this article as:
Kubo, N., Salomon, B., Komatsuda, T. et al. Theor Appl Genet (2005) 110: 995. doi:10.1007/s00122-004-1839-x

Abstract

The mitochondrial rps2 gene from barley, like that of rice, wheat, and maize, has an extended open reading frame (ORF) at the 3′-region when compared to that from lower plants. However, the extended portions are variable among these cereals. Since barley and wheat belong to the same tribe (Triticeae), it would be interesting to know when and where the two types of rps2 were generated during evolution. To determine this, we utilized the mitochondrial (mt)DNA sequence to examine variations of the rps2 genes in the tribe Triticeae. By means of the variable 3′-region, the distribution of barley (B)-type and wheat (W)-type rps2 sequences was studied in 19 genera of the tribe. The B-type sequence was identified in 10 of the 19 genera, whereas the W-type sequence was present in all 19 genera. Thus, ten of the examined genera have both types of rps2 sequences due to the presence of two copies of the gene. The W-type sequence was also present in the tribe Bromeae and the B-type sequence was also found in Aveneae and Poeae. Phylogenetic trees based on the B-type and W-type sequences were different from those based on other molecular data. This suggests that the mitochondrial genome in Triticeae has a unique evolutionary history.

Supplementary material

122_2004_1839_ESM_supp.pdf (27 kb)
Supplementary Fig. 1 Nucleotide sequence alignment of mitochondrial rps2 sequences from Triticeae and the other related taxa. (A) Alignment of the barley (B)-type sequences. (B) Alignment of the wheat (W)-type sequences. Phylogenetically informative sites are indicated by asterisks (*) below the nucleotide sequence. Gaps are shown by dots. (PDF 28 KB)

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • N. Kubo
    • 1
    • 3
  • B. Salomon
    • 2
  • T. Komatsuda
    • 1
  • R. von Bothmer
    • 2
  • K. Kadowaki
    • 1
  1. 1.Genetic Diversity DepartmentNational Institute of Agrobiological SciencesTsukubaJapan
  2. 2.Department of Crop ScienceThe Swedish University of Agricultural SciencesAlnarpSweden
  3. 3.Graduate School of Agriculture, Kyoto Prefectural UniversityKyoto Prefectural Institute of Agricultural BiotechnologySeika, Soraku 619-0244Japan