Theoretical and Applied Genetics

, Volume 110, Issue 1, pp 1–11

Identification and characterization of a QTL on chromosome 2 for cytosolic glutamine synthetase content and panicle number in rice

  • Mitsuhiro Obara
  • Tadashi Sato
  • Shohei Sasaki
  • Kenji Kashiba
  • Atsushi Nagano
  • Ikuo Nakamura
  • Takeshi Ebitani
  • Masahiro Yano
  • Tomoyuki Yamaya
Original Paper

DOI: 10.1007/s00122-004-1828-0

Cite this article as:
Obara, M., Sato, T., Sasaki, S. et al. Theor Appl Genet (2004) 110: 1. doi:10.1007/s00122-004-1828-0

Abstract

A quantitative trait locus (QTL) associated with the protein content of cytosolic glutamine synthetase (GS1; EC 6.3.1.2) in senescing leaves, panicle number, and panicle weight was characterized in rice (Oryza sativa L.). A near-isogenic line (NIL), C-22, developed by marker-assisted selection was grown under different nitrogen levels in the greenhouse and in a paddy field. Chromosome 2 of C-22 had an approximately 50-cM segment substituted from the Kasalath (indica) chromosome in a Koshihikari (japonica) genetic background. C-22 showed a 12–37% lower content of GS1 protein in leaf blades than Koshihikari, which was in good agreement with a QTL region positively affected by the japonica chromosome. At an early vegetative stage, C-22 had more active tillers than Koshihikari in the greenhouse. At the reproductive stage, both panicle number and total panicle weight of C-22 were significantly higher than those of Koshihikari, particularly when the plants were grown under a low-nitrogen condition. These traits of C-22 were further confirmed in a paddy field. Thus, tiller development was positively affected by the Kasalath chromosome at an early vegetative stage, which resulted in an increased panicle number and panicle weight at the mature stage in C-22. These data indicate that the target QTL (Pnn1; panicle number 1) is important in the development of tillers and panicles in rice. Linkage analyses for panicle number and ratio of developing tiller formation in the second axil (RDT) revealed that Pnn1 was delimited at the 6.7-cM region.

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Mitsuhiro Obara
    • 1
    • 2
  • Tadashi Sato
    • 3
  • Shohei Sasaki
    • 1
  • Kenji Kashiba
    • 1
  • Atsushi Nagano
    • 1
  • Ikuo Nakamura
    • 4
  • Takeshi Ebitani
    • 5
  • Masahiro Yano
    • 6
  • Tomoyuki Yamaya
    • 1
    • 7
  1. 1.Graduate School of Agricultural ScienceTohoku UniversitySendaiJapan
  2. 2.Research Fellow of the Japan Society for the Promotion of ScienceChiyodaJapan
  3. 3.Graduate School of Life SciencesTohoku UniversitySendaiJapan
  4. 4.Graduate School of Science and TechnologyChiba UniversityChibaJapan
  5. 5.Toyama Agricultural Research CenterToyamaJapan
  6. 6.Department of Molecular GeneticsNational Institute of Agrobiological Sciences IbarakiJapan
  7. 7.Plant Science CenterRIKENYokohamaJapan