Theoretical and Applied Genetics

, Volume 109, Issue 6, pp 1125–1132

Use of 2n gametes for the production of sexual polyploids from sterile Oriental × Asiatic hybrids of lilies (Lilium)

Authors

  • R. Barba-Gonzalez
    • Plant Research InternationalWageningen University and Research Centre
  • A. C. Lokker
    • Plant Research InternationalWageningen University and Research Centre
  • K.-B. Lim
    • Genomics Division, National Institute of Agricultural Biotechnology (NIAB)RDA
  • M. S. Ramanna
    • Plant Research InternationalWageningen University and Research Centre
    • Plant Research InternationalWageningen University and Research Centre
Original Paper

DOI: 10.1007/s00122-004-1739-0

Cite this article as:
Barba-Gonzalez, R., Lokker, A.C., Lim, K. et al. Theor Appl Genet (2004) 109: 1125. doi:10.1007/s00122-004-1739-0

Abstract

Sixteen Oriental and 12 Asiatic cultivars were crossed in 158 different combinations. A total of 708 F1 hybrids were obtained from 86 of the different combinations of 15 Oriental and 11 Asiatic cultivars. Because the Lilium cultivars (2n=2x=24) used for the production of these hybrids belong to two different taxonomic sections—Archelirion (O) and Sinomartagon (A), respectively—the F1 hybrids (OA) could be obtained only through embryo, embryo sac rescue, ovary slice or ovule culture. Most of the F1 hybrids were highly sterile (did not produce viable n gametes) due to the failure of chromosome pairing. However, in a few cases F1 plants were found that produced viable 2n pollen at variable frequencies. These 2n pollen grains were successfully used for the production of backcross progenies. Using genomic in situ hybridization we found intergenomic recombinant chromosomes in the sexual polyploid progenies. These results indicate that there are effective prospects for combining important horticultural traits from the two main groups of cultivars of lilies through sexual polyploidization.

Copyright information

© Springer-Verlag 2004