Theoretical and Applied Genetics

, Volume 108, Issue 5, pp 903–913

Characterization of soybean genomic features by analysis of its expressed sequence tags

  • Ai-Guo Tian
  • Jun Wang
  • Peng Cui
  • Yu-Jun Han
  • Hao Xu
  • Li-Juan Cong
  • Xian-Gang Huang
  • Xiao-Ling Wang
  • Yong-Zhi Jiao
  • Bang-Jun Wang
  • Yong-Jun Wang
  • Jin-Song Zhang
  • Shou-Yi Chen
Original Paper

DOI: 10.1007/s00122-003-1499-2

Cite this article as:
Tian, AG., Wang, J., Cui, P. et al. Theor Appl Genet (2004) 108: 903. doi:10.1007/s00122-003-1499-2

Abstract

We analyzed 314,254 soybean expressed sequence tags (ESTs), including 29,540 from our laboratory and 284,714 from GenBank. These ESTs were assembled into 56,147 unigenes. About 76.92% of the unigenes were homologous to genes from Arabidopsis thaliana (Arabidopsis). The putative products of these unigenes were annotated according to their homology with the categorized proteins of Arabidopsis. Genes corresponding to cell growth and/or maintenance, enzymes and cell communication belonged to the slow-evolving class, whereas genes related to transcription regulation, cell, binding and death appeared to be fast-evolving. Soybean unigenes with no match to genes within the Arabidopsis genome were identified as soybean-specific genes. These genes were mainly involved in nodule development and the synthesis of seed storage proteins. In addition, we also identified 61 genes regulated by salicylic acid, 1,322 transcription factor genes and 326 disease resistance-like genes from soybean unigenes. SSR analysis showed that the soybean genome was more complex than the Arabidopsis and the Medicago truncatula genomes. GC content in soybean unigene sequences is similar to that in Arabidopsis and M. truncatula. Furthermore, the combined analysis of the EST database and the BAC-contig sequences revealed that the total gene number in the soybean genome is about 63,501.

Supplementary material

Supplement 1

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(PDF 525 KB)

Supplement 2

supplement2.pdf (187 kb)
(PDF 187 KB)

Supplement 3

supplement3.pdf (152 kb)
(PDF 153 KB)

Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • Ai-Guo Tian
    • 1
  • Jun Wang
    • 2
  • Peng Cui
    • 2
  • Yu-Jun Han
    • 2
  • Hao Xu
    • 2
  • Li-Juan Cong
    • 2
  • Xian-Gang Huang
    • 2
  • Xiao-Ling Wang
    • 2
  • Yong-Zhi Jiao
    • 2
  • Bang-Jun Wang
    • 1
  • Yong-Jun Wang
    • 1
  • Jin-Song Zhang
    • 1
  • Shou-Yi Chen
    • 1
  1. 1.Plant Biotechnology Laboratory, Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijingChina
  2. 2.Beijing Genomics InstituteChinese Academy of SciencesBeijingChina