Theoretical and Applied Genetics

, Volume 107, Issue 7, pp 1201–1207

High variability and disomic segregation of microsatellites in the octoploid Fragaria virginiana Mill. (Rosaceae)

  • M. V. Ashley
  • J. A. Wilk
  • S. M. N. Styan
  • K. J. Craft
  • K. L. Jones
  • K. A. Feldheim
  • K. S. Lewers
  • T. L. Ashman

DOI: 10.1007/s00122-003-1370-5

Cite this article as:
Ashley, M.V., Wilk, J.A., Styan, S.M.N. et al. Theor Appl Genet (2003) 107: 1201. doi:10.1007/s00122-003-1370-5

Abstract

The objectives of the present study were to develop microsatellite markers for the wild strawberry, Fragaria virginiana, to evaluate segregation patterns of microsatellite alleles in this octoploid species, and assess genetic variability at microsatellite loci in a wild population. A genomic library was screened for microsatellite repeats and several PCR primers were designed and tested. We also tested the use of heterologous primers and found that F. virginiana primers amplified products in cultivated strawberry, Fragaria × ananassa Duch. and Fragaria chiloensis. Similarly, microsatellite loci developed from cultivated strawberry also successfully amplified F. virginiana loci. We investigated four microsatellite loci in detail, three developed from F. virginiana and one from cultivated strawberry. A survey of 100 individuals from a population of F. virginiana in Pennsylvania demonstrated high heterozygosities (He or gene diversity ranged from 0.80 to 0.88 per locus) and allelic diversity (12–17 alleles per locus), but individual plants had no more than two alleles per locus. Segregation patterns in parents and progeny of two controlled crosses at these four loci were consistent with disomic Mendelian inheritance. Together these findings suggest that the genome of F. virginiana is "highly diploidized" and at least a subset of microsatellite loci can be treated as codominant, diploid markers. Significant heterozygote deficiencies were found at three of the four loci for hermaphroditic individuals but for only one locus among females in this gynodioecious species.

Keywords

Fragaria virginianaWild strawberryMicrosatellitesPolyploidOctoploidFragaria × ananassa

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • M. V. Ashley
    • 1
  • J. A. Wilk
    • 1
  • S. M. N. Styan
    • 2
    • 4
  • K. J. Craft
    • 1
  • K. L. Jones
    • 1
  • K. A. Feldheim
    • 1
  • K. S. Lewers
    • 2
  • T. L. Ashman
    • 3
  1. 1.Department of Biological SciencesUniversity of Illinois at ChicagoChicagoUSA
  2. 2.USDA-ARS Fruit LabBld 010A, BARC-WBeltsvilleUSA
  3. 3.Department of Biological SciencesUniversity of PittsburghPittsburghUSA
  4. 4.Pioneer Hi-Bred International Incorporated. A DuPont CompanyWaimeaUSA