Theoretical and Applied Genetics

, Volume 107, Issue 6, pp 965–971

Mapping of Rym14Hb, a gene introgressed from Hordeum bulbosum and conferring resistance to BaMMV and BaYMV in barley

  • B. Ruge
  • A. Linz
  • R. Pickering
  • G. Proeseler
  • P. Greif
  • P. Wehling
Article

DOI: 10.1007/s00122-003-1339-4

Cite this article as:
Ruge, B., Linz, A., Pickering, R. et al. Theor Appl Genet (2003) 107: 965. doi:10.1007/s00122-003-1339-4

Abstract.

Hordeum bulbosum represents the secondary gene pool of barley and constitutes a potential source of various disease resistances in barley breeding. Interspecific crosses of H. vulgare × H. bulbosum resulted in recombinant diploid-barley progeny with immunity to BaMMV after mechanical inoculation. Tests on fields contaminated with different viruses demonstrated that resistance was effective against all European viruses of the soil-borne virus complex (BaMMV, BaYMV-1, -2). Genetic analysis revealed that resistance was dominantly inherited. Marker analysis in a F5 mapping family was performed to map the introgression in the barley genome and to estimate its size after several rounds of recombination. RFLP anchor-marker alleles indicative of an H. bulbosum introgression were found to cover an interval 2.9 cM in length on chromosome 6HS. The soil-borne virus resistance locus harboured by this introgressed segment was designated Rym14Hb. For marker-assisted selection of Rym14Hb carriers, a diagnostic codominant STS marker was derived from an AFLP fragment amplified from leaf cDNA of homozygous-resistant genotypes inoculated with BaMMV.

Keywords.

Soil-borne virus resistanceHordeum bulbosumHordeum vulgareIntrogression mappingRym14Hb

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • B. Ruge
    • 1
  • A. Linz
    • 1
  • R. Pickering
    • 2
  • G. Proeseler
    • 3
  • P. Greif
    • 4
  • P. Wehling
    • 1
  1. 1.Federal Centre for Breeding Research on Cultivated Plants, Institute of Agricultural Crops, Rudolf-Schick-Platz 3a, D-18190 Groß Lüsewitz, Germany
  2. 2.New Zealand Institute for Crop and Food Research Limited, Private Bag 4704, Christchurch, New Zealand
  3. 3.Federal Centre for Breeding Research on Cultivated Plants, Institute of Epidemiology and Resistance Resources, Aschersleben, Germany
  4. 4.Saatzuchtgesellschaft Streng's Erben GmbH and Co. KG, Uffenheim, Germany