Theoretical and Applied Genetics

, Volume 107, Issue 4, pp 705–712

Gene flow estimation with microsatellites in a Malagasy seed orchard of Eucalyptus grandis

  • G. Chaix
  • S. Gerber
  • V. Razafimaharo
  • P. Vigneron
  • D. Verhaegen
  • S. Hamon
Article

DOI: 10.1007/s00122-003-1294-0

Cite this article as:
Chaix, G., Gerber, S., Razafimaharo, V. et al. Theor Appl Genet (2003) 107: 705. doi:10.1007/s00122-003-1294-0

Abstract

Eucalyptus grandis has a mixed-mating reproductive system. Malagasy Eucalyptus seed orchards were established 15 years ago with two aims both based on panmixia: open-pollinated seed production and genetic improvement. The panmixia hypothesis has never been confirmed in the seed orchard. From a seedling seed-orchard stand comprising 349 trees and using data obtained with six selected microsatellite markers, paternity analysis was performed for 724 offspring collected on 30 adult trees. Paternity assignment, based on exclusion procedures and likelihood-ratio method, was achieved with high accuracy; the exclusion probability value was 0.997. The outcrossing rate was very high (96.7%). More than 50% of potential male trees (199 out of 349) in the seed orchard contributed to pollination for 440 offspring of 30 progenies (8.6% of the basic population). The pollination rate from outside the seed orchard was high (39.2%), but might be due to the small size of this seed orchard. This study showed that "panmixia-like pollination" can be assumed.

Keywords

Eucalyptus grandis Pollen flow Seed orchard Paternity analysis Microsatellite Madagascar 

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • G. Chaix
    • 1
  • S. Gerber
    • 2
  • V. Razafimaharo
    • 1
  • P. Vigneron
    • 3
  • D. Verhaegen
    • 3
  • S. Hamon
    • 4
  1. 1.Cirad-Forêt/Fofifa, B.P. 745, Antananarivo 101, Madagascar
  2. 2.INRA, Laboratoire de génétique et amélioration des arbres forestiers, BP 45, F-33611 Gazinet Cedex, France
  3. 3.Cirad-Forêt, Campus de Baillarguet TA 10C, 34398 Montpellier Cedex 5 France
  4. 4.IRD Montpellier, 911 Avenue Agropolis, 34032 Montpellier Cedex France

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