Theoretical and Applied Genetics

, Volume 105, Issue 6, pp 870–877

Expression of wild-type pinB sequence in transgenic wheat complements a hard phenotype

  •  B. Beecher
  •  A. Bettge
  •  E. Smidansky
  •  M. Giroux

DOI: 10.1007/s00122-002-1034-x

Cite this article as:
Beecher, B., Bettge, A., Smidansky, E. et al. Theor Appl Genet (2002) 105: 870. doi:10.1007/s00122-002-1034-x

Abstract.

Wheat grain hardness is a major factor in the wheat end-product quality. Grain hardness in wheat affects such parameters as milling yield, starch damage and baking properties. A single locus determines whether wheat is hard or soft textured. This locus, termed Hardness (Ha), resides on the short arm of chromosome 5D. Sequence alterations in the tryptophan-rich proteins puroindoline a and b (PINA and PINB) are inseparably linked to hard textured grain, but their role in endosperm texture has been controversial. Here, we show that the pinB-D1b alteration, common in hard textured wheats, can be complemented by the expression of wild-type pinB-D1a in transformed plants. Transgenic wheat seeds expressing wild-type pinB were soft in phenotype, having greatly increased friabilin levels, and greatly decreased kernel hardness and damaged starch. These results indicate that the pinB-D1b alteration is most likely the causative Ha mutation in the majority of hard wheats.

Wheat Endosperm texture Grain hardness Puroindolines

Copyright information

© Springer 2002

Authors and Affiliations

  •  B. Beecher
    • 1
  •  A. Bettge
    • 3
  •  E. Smidansky
    • 1
  •  M. Giroux
    • 1
  1. 1.Dept. of Plant Sciences and Plant Pathology, Ag. BioSci. Fac., Montana State University, Bozeman, MT 59717-3150, USA
  2. 2.Present address: Department of Agronomy and Horticulture, University of Nebraska, 279 Plant Sciences, Lincoln, NE 68583-0915, USA
  3. 3.USDA-ARS Wheat Quality Lab, Wheat Genetics, Quality, Physiology and Disease Research Unit, E. 202 FSHN, Washington State University, Pullman, WA 99164, USA