Theoretical and Applied Genetics

, Volume 104, Issue 6, pp 981–986

Mapping quantitative trait loci controlling seed longevity in rice (Oryza sativa L.)

  •  K. Miura
  •  S. Lin
  •  M. Yano
  •  T. Nagamine

DOI: 10.1007/s00122-002-0872-x

Cite this article as:
Miura, K., Lin, S., Yano, M. et al. Theor Appl Genet (2002) 104: 981. doi:10.1007/s00122-002-0872-x

Abstract.

Quantitative trait loci (QTLs) controlling seed longevity in rice were identified using 98 backcross inbred lines (BILs) derived from a cross between a japonica variety Nipponbare and an indica variety Kasalath. Seeds of each BIL were kept for 12 months at 30 °C in dry conditions to promote loss of viability. To measure seed longevity, we performed an additional aging-processing treatment for 2 months at 30 °C maintaining seeds at 15% moisture content. We measured the germination percent of these treated seeds at 25 °C for 7 days as the degree of seed longevity. The germination of BILs ranged from 0 to 100% with continuous variation. Three putative QTLs for seed longevity, qLG-2, qLG-4 and qLG-9, were detected on chromosome 2, 4 and 9, respectively. Kasalath alleles increased the seed longevity at these QTLs. The QTL with the largest effect, qLG-9, explained 59.5% of total phenotypic variation in BILs. The other two QTLs, qLG-2 and qLG-4, explained 13.4 and 11.6% of the total phenotypic variation, respectively. We also verified the effect of the Kasalath allele of qLG-9 using chromosome segment substitution lines. Furthermore, QTLs for seed dormancy were identified on chromosomes 1, 3, 5, 7 and 11. Based on the comparison of the chromosomal location of QTLs for seed longevity and seed dormancy, these traits seem to be controlled by different genetic factors.

Oryza sativa L. Seed longevity Seed dormancy QTL analysis Backcross inbred lines Chromosome segment substitution lines 

Copyright information

© Springer-Verlag 2002

Authors and Affiliations

  •  K. Miura
    • 1
  •  S. Lin
    • 3
  •  M. Yano
    • 2
  •  T. Nagamine
    • 1
  1. 1.Gene Bank, National Institute of Agrobiological Sciences, 2-1-2, Kannondai, Tsukuba, Ibaraki 305-8602, Japan
  2. 2.Molecular Genetics Department, National Institute of Agrobiological Sciences, 2-1-2, Kannondai, Tsukuba, Ibaraki 305-8602, Japan
  3. 3.Institute of the Society for Techno-innovation of Agriculture, Forestry and Fisheries, 446-1, Ippaizuka, Kamiyokoba, Tsukuba, Ibaraki 305-0854, Japan
  4. 4.Present address: Honda R & D Co., Ltd. Creationcore Kazusa, 1688 Kamisawata, Yana, Kisarazu, Chiba 292-0812, Japan

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