Theoretical and Applied Genetics

, Volume 104, Issue 5, pp 772–778

Genetic dissection of a genomic region for a quantitative trait locus, Hd3, into two loci, Hd3a and Hd3b, controlling heading date in rice

Authors

  •  L. Monna
    • Institute of the Society for Techno-innovation of Agriculture, Forestry and Fisheries, Tsukuba, Ibaraki 305-0854, Japan
  •  H. Lin
    • Bio-oriented Technology Research Advancement Institution, Omiya, Saitama 331-8537, Japan
  •  S. Kojima
    • Institute of the Society for Techno-innovation of Agriculture, Forestry and Fisheries, Tsukuba, Ibaraki 305-0854, Japan
  •  T. Sasaki
    • Department of Molecular Genetics, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan
  •  M. Yano
    • Department of Molecular Genetics, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan

DOI: 10.1007/s00122-001-0813-0

Cite this article as:
Monna, L., Lin, H., Kojima, S. et al. Theor Appl Genet (2002) 104: 772. doi:10.1007/s00122-001-0813-0

Abstract.

The rice photoperiod sensitivity gene Hd3 was originally detected as a heading date-related quantitative trait locus localized on chromosome 6 of rice. High-resolution linkage mapping of Hd3 was performed using a large segregating population derived from advanced backcross progeny between a japonica variety, Nipponbare, and an indica variety, Kasalath. To determine the genotype of Hd3, we employed progeny testing under natural field and short-day conditions. As a result, two tightly linked loci, Hd3a and Hd3b, were identified in the Hd3 region. Nearly-isogenic lines for Hd3a and Hd3b were selected from progeny using marker-assisted selection. The inheritance mode of both Hd3a and Hd3b was found to be additive. Analysis of daylength response in nearly-isogenic lines of Hd3a and Hd3b showed that the Kasalath allele at Hd3a promotes heading under short-day conditions while that at Hd3b causes late heading under long-day and natural field conditions.

Oryza sativa L. QTL Photoperiod sensitivity Nearly-isogenic line Linkage analysis

Copyright information

© Springer-Verlag 2002