Naturwissenschaften

, Volume 100, Issue 2, pp 193–197

Genomewide analysis indicates that queen larvae have lower methylation levels in the honey bee (Apis mellifera)

  • Yuan Yuan Shi
  • Wei Yu Yan
  • Zachary Y. Huang
  • Zi Long Wang
  • Xiao Bo Wu
  • Zhi Jiang Zeng
Short Communication

DOI: 10.1007/s00114-012-1004-3

Cite this article as:
Shi, Y.Y., Yan, W.Y., Huang, Z.Y. et al. Naturwissenschaften (2013) 100: 193. doi:10.1007/s00114-012-1004-3

Abstract

The honey bee is a social insect characterized by caste differentiation, by which a young larva can develop into either a queen or a worker. Despite possessing the same genome, queen and workers display marked differences in reproductive capacity, physiology, and behavior. Recent studies have shown that DNA methylation plays important roles in caste differentiation. To further explore the roles of DNA methylation in this process, we analyzed DNA methylome profiles of both queen larvae (QL) and worker larvae (WL) of different ages (2, 4, and 6 day old), by using methylated DNA immunoprecipitation-sequencing (meDIP-seq) technique. The global DNA methylation levels varied between the larvae of two castes. DNA methylation increased from 2-day- to 4-day-old QL and then decreased in 6-day-old larvae. In WL, methylation levels increased with age. The methylcytosines in both larvae were enriched in introns, followed by coding sequence (CDS) regions, CpG islands, 2 kbp downstream and upstream of genes, and 5′ and 3′ untranslated regions (UTRs). The number of differentially methylated genes (DMGs) in 2-, 4-, and 6-day-old QL and WL was 725, 3,013, and 5,049, respectively. Compared to 4- and 6-day-old WL, a large number of genes in QL were downmethylated, which were involved in many processes including development, reproduction, and metabolic regulation. In addition, some DMGs were concerned with caste differentiation.

Keywords

Honey beeQueen larvaeWorker larvaeDNA methylationDifferentially methylated genesCaste differentiation

Supplementary material

114_2012_1004_MOESM1_ESM.doc (484 kb)
ESM 1(DOC 484 kb)

Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Yuan Yuan Shi
    • 1
  • Wei Yu Yan
    • 1
  • Zachary Y. Huang
    • 2
  • Zi Long Wang
    • 1
  • Xiao Bo Wu
    • 1
  • Zhi Jiang Zeng
    • 1
  1. 1.Honeybee Research InstituteJiangxi Agricultural UniversityNanchangPeople’s Republic of China
  2. 2.Department of EntomologyMichigan State UniversityEast LansingUSA