Naturwissenschaften

, Volume 96, Issue 2, pp 267–278

Preservation of ancient DNA in thermally damaged archaeological bone

  • Claudio Ottoni
  • Hannah E. C. Koon
  • Matthew J. Collins
  • Kirsty E. H. Penkman
  • Olga Rickards
  • Oliver E. Craig
Original Paper

DOI: 10.1007/s00114-008-0478-5

Cite this article as:
Ottoni, C., Koon, H.E.C., Collins, M.J. et al. Naturwissenschaften (2009) 96: 267. doi:10.1007/s00114-008-0478-5

Abstract

Evolutionary biologists are increasingly relying on ancient DNA from archaeological animal bones to study processes such as domestication and population dispersals. As many animal bones found on archaeological sites are likely to have been cooked, the potential for DNA preservation must be carefully considered to maximise the chance of amplification success. Here, we assess the preservation of mitochondrial DNA in a medieval cattle bone assemblage from Coppergate, York, UK. These bones have variable degrees of thermal alterations to bone collagen fibrils, indicative of cooking. Our results show that DNA preservation is not reliant on the presence of intact collagen fibrils. In fact, a greater number of template molecules could be extracted from bones with damaged collagen. We conclude that moderate heating of bone may enhance the retention of DNA fragments. Our results also indicate that ancient DNA preservation is highly variable, even within a relatively recent assemblage from contexts conducive to organic preservation, and that diagenetic parameters based on protein diagenesis are not always useful for predicting ancient DNA survival.

Keywords

Ancient DNA Cooking Collagen Bone diagenesis 

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Claudio Ottoni
    • 1
  • Hannah E. C. Koon
    • 2
  • Matthew J. Collins
    • 2
  • Kirsty E. H. Penkman
    • 3
  • Olga Rickards
    • 1
  • Oliver E. Craig
    • 1
    • 4
  1. 1.Centro di Antropologia Molecolare per lo studio del DNA antico, Dipartimento di BiologiaUniversità di Roma “Tor Vergata”RomeItaly
  2. 2.BioArCh, Department of BiologyUniversity of YorkYorkUK
  3. 3.BioArCh, Department of ChemistryUniversity of YorkYorkUK
  4. 4.Department of ArchaeologyUniversity of YorkThe King’s Manor YorkUK