Naturwissenschaften

, Volume 94, Issue 1, pp 61–64

Antennally mediated negative feedback regulation of pheromone production in the pine engraver beetle, Ips pini

  • Matthew D. Ginzel
  • Jeremy C. Bearfield
  • Christopher I. Keeling
  • Colin C. McCormack
  • Gary J. Blomquist
  • Claus Tittiger
Short Communication

DOI: 10.1007/s00114-006-0158-2

Cite this article as:
Ginzel, M.D., Bearfield, J.C., Keeling, C.I. et al. Naturwissenschaften (2007) 94: 61. doi:10.1007/s00114-006-0158-2

Abstract

Bark beetles use monoterpenoid aggregation pheromones to coordinate host colonization and mating. These chemical signals are produced de novo in midgut cells via the mevalonate pathway, and pheromone production may be regulated by a negative feedback system mediated through the antennae. In this study, we explored the effect of antennectomy on pheromone production and transcript levels of key mevalonate pathway genes in juvenile hormone III-treated male pine engraver beetles, Ips pini (Say). Antennectomized males produced significantly greater amounts of pheromone than podectomized males and those with intact antennae. Likewise, mRNA levels of three mevalonate pathway genes important in pheromone biosynthesis were measured by quantitative real-time PCR and found to be induced to a greater extent with antennectomy, suggesting a transcriptional regulation of pheromone production.

Keywords

Ips piniPheromonesAntennectomyJuvenile hormoneMevalonate pathway

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • Matthew D. Ginzel
    • 1
    • 2
  • Jeremy C. Bearfield
    • 1
  • Christopher I. Keeling
    • 1
    • 3
  • Colin C. McCormack
    • 1
  • Gary J. Blomquist
    • 1
  • Claus Tittiger
    • 1
  1. 1.Department of Biochemistry and Molecular BiologyUniversity of NevadaRenoUSA
  2. 2.Department of Entomology and Department of Forestry & Natural Resources, Hardwood Tree Improvement and Regeneration CenterPurdue UniversityWest LafayetteUSA
  3. 3.Michael Smith LaboratoriesUniversity of British ColumbiaVancouverCanada