Short Communication

Naturwissenschaften

, Volume 94, Issue 1, pp 61-64

First online:

Antennally mediated negative feedback regulation of pheromone production in the pine engraver beetle, Ips pini

  • Matthew D. GinzelAffiliated withDepartment of Biochemistry and Molecular Biology, University of NevadaDepartment of Entomology and Department of Forestry & Natural Resources, Hardwood Tree Improvement and Regeneration Center, Purdue University Email author 
  • , Jeremy C. BearfieldAffiliated withDepartment of Biochemistry and Molecular Biology, University of Nevada
  • , Christopher I. KeelingAffiliated withDepartment of Biochemistry and Molecular Biology, University of NevadaMichael Smith Laboratories, University of British Columbia
  • , Colin C. McCormackAffiliated withDepartment of Biochemistry and Molecular Biology, University of Nevada
  • , Gary J. BlomquistAffiliated withDepartment of Biochemistry and Molecular Biology, University of Nevada
  • , Claus TittigerAffiliated withDepartment of Biochemistry and Molecular Biology, University of Nevada

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Bark beetles use monoterpenoid aggregation pheromones to coordinate host colonization and mating. These chemical signals are produced de novo in midgut cells via the mevalonate pathway, and pheromone production may be regulated by a negative feedback system mediated through the antennae. In this study, we explored the effect of antennectomy on pheromone production and transcript levels of key mevalonate pathway genes in juvenile hormone III-treated male pine engraver beetles, Ips pini (Say). Antennectomized males produced significantly greater amounts of pheromone than podectomized males and those with intact antennae. Likewise, mRNA levels of three mevalonate pathway genes important in pheromone biosynthesis were measured by quantitative real-time PCR and found to be induced to a greater extent with antennectomy, suggesting a transcriptional regulation of pheromone production.

Keywords

Ips pini Pheromones Antennectomy Juvenile hormone Mevalonate pathway