Journal of Molecular Medicine

, Volume 79, Issue 11, pp 631–640

T cell receptor excision circles as markers for recent thymic emigrants: basic aspects, technical approach, and guidelines for interpretation

  • Mette D. Hazenberg
  • Martie C. Verschuren
  • Dörte Hamann
  • Frank Miedema
  • Jacques J. Dongen
Original Article

DOI: 10.1007/s001090100271

Cite this article as:
Hazenberg, M.D., Verschuren, M.C., Hamann, D. et al. J Mol Med (2001) 79: 631. doi:10.1007/s001090100271

Abstract

T cell differentiation in the thymus is characterized by a hierarchical order of rearrangement steps in the T cell receptor (TCR) genes, resulting in the joining of V, D, and J gene segments. During each of the rearrangement steps, DNA fragments between rearranging V, D, and J gene segments are deleted as circular excision products, the so-called TRECs (T cell receptor excision circles). TRECs are assumed to have a high over-time stability, but they can not multiply and consequently are diluted during T cell proliferation. It was recently suggested that quantitative detection of TRECs would allow for direct measurement of thymic output. The δRec-ψJα TREC appears to be the best marker, because the majority of thymocyte expansion occurs before this TREC is formed. However, apart from thymic output several other factors determine the TREC content of a T cell population, such as cell division and cell death. Likewise, the number of TRECs depends not only on thymic output, but also on the longevity of naive T cells. This warrants caution with regard to the interpretation of TREC data as measured in healthy and diseased individuals. δRec-ψJα TREC detection is a new and elegant tool for identification of recent thymic emigrants in the periphery, but further research is required for making quantitative estimations of thymic output with the use of TREC analysis.

TREC Thymus T cell receptor gene rearrangements Human immunodeficiency virus Reconstitution

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Mette D. Hazenberg
    • 1
  • Martie C. Verschuren
    • 2
  • Dörte Hamann
    • 1
  • Frank Miedema
    • 1
  • Jacques J. Dongen
    • 2
  1. 1.Department of Clinical Viro-Immunology, CLB, and Laboratory for Experimental and Clinical Immunology, Academic Medical Center, University of Amsterdam, Plesmanlaan 125, 1066 CX AmsterdamThe Netherlands
  2. 2.Department of Immunology, Erasmus University Rotterdam/University Hospital Rotterdam, RotterdamThe Netherlands