Journal of Molecular Medicine

, Volume 78, Issue 10, pp 554–561

Serum levels of leptin and proinflammatory cytokines in patients with advanced-stage cancer at different sites

  • Giovanni Mantovani
  • Antonio Macciò
  • Loredana Mura
  • Elena Massa
  • Maria Caterina Mudu
  • Carlo Mulas
  • Maria Rita Lusso
  • Clelia Madeddu
  • Annita Dessì
Original Article

DOI: 10.1007/s001090000137

Cite this article as:
Mantovani, G., Macciò, A., Mura, L. et al. J Mol Med (2000) 78: 554. doi:10.1007/s001090000137

Abstract

Leptin is a recently identified hormone produced by the adipocyte ob gene which acts as a negative feedback signal critical to the normal control of food intake and body weight. A number of proinflammatory cytokines, such as interleukin (IL) 1α, IL-6, tumor necrosis factor (TNF) α and interferon (IFN) γ, have been proposed as mediators of cancer cachexia. These data suggest that abnormalities in leptin production/release or in its feedback mechanism play a role in cancer patients. To elucidate this we studied the relationship between total serum leptin and serum cytokines IL-1α, IL-6, TNFα as well as the production of leptin and cytokines by peripheral blood mononuclear cells (PBMC) isolated from cancer patients. Sixteen advanced cancer patients (mainly stage IV) with tumors at different sites were included in the study. The serum levels of leptin in cancer patients were significantly lower than those of healthy individuals at all times (7 a.m., noon, 3 p.m.). No significant differences were found in circadian rhythm between patients and controls. Serum levels of IL-1α, IL-6, and TNFα were significantly higher in cancer patients than in healthy individuals. An inverse correlation between serum levels of leptin and IL-6 was found in cancer patients. The production in culture of leptin by unstimulated PBMCs and those stimulated by phytohemagglutinin M or by phorbol myristate acetate isolated from cancer patients was very low; no differences were observed in comparison with leptin production by PBMCs from healthy individuals.

Leptin Proinflammatory cytokines Cancer anorexia/cachexia Nutritional status Immunological aspects

Copyright information

© Springer-Verlag 2000

Authors and Affiliations

  • Giovanni Mantovani
    • 1
  • Antonio Macciò
    • 2
  • Loredana Mura
    • 1
  • Elena Massa
    • 1
  • Maria Caterina Mudu
    • 1
  • Carlo Mulas
    • 1
  • Maria Rita Lusso
    • 1
  • Clelia Madeddu
    • 1
  • Annita Dessì
    • 3
  1. 1.Department of Medical Oncology, University of Cagliari, Via S. Giorgio 12, 09124 CagliariItaly
  2. 2.Division of Obstetrics and Gynecology, Carbonia Hospital, CarboniaItaly
  3. 3.Department of Obstetrics and Gynecology, University of Cagliari, Via S. Giorgio 12, 09124 CagliariItaly