Journal of Molecular Medicine

, Volume 78, Issue 7, pp 389–394

Molecular cloning and expression of a novel klotho-related protein

Authors

  • Kensei Yahata
    • Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Kyoto 606-8507
  • Kiyoshi Mori
    • Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Kyoto 606-8507
  • Hiroshi Arai
    • Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Kyoto 606-8507
  • Susumu Koide
    • Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Kyoto 606-8507
  • Yoshihiro Ogawa
    • Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Kyoto 606-8507
  • Masashi Mukoyama
    • Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Kyoto 606-8507
  • Akira Sugawara
    • Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Kyoto 606-8507
  • Shoichi Ozaki
    • Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Kyoto 606-8507
  • Issei Tanaka
    • Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Kyoto 606-8507
  • Yo-ichi Nabeshima
    • Department of Pathology and Tumor Biology, Faculty of Medicine, Kyoto University Graduate School of Medicine, Kyoto 606-8501
  • Kazuwa Nakao
    • Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Kyoto 606-8507
Original Article

DOI: 10.1007/s001090000131

Cite this article as:
Yahata, K., Mori, K., Arai, H. et al. J Mol Med (2000) 78: 389. doi:10.1007/s001090000131

Abstract

Klotho protein is a novel β-glucosidase-like protein produced predominantly in the kidney. The klotho mouse, which genetically lacks klotho gene expression, manifests various systemic phenotypes resembling aging. In the present study we succeeded in isolating a novel human protein structurally related to klotho protein. The protein possesses one β-glucosidase-like domain and is 42% identical with klotho protein at the amino acid level. Unlike klotho protein, it possesses neither a signal sequence nor a transmembrane domain, suggesting that it is a cytosolic protein, and thus was termed cytosolic β-glucosidase-like protein-1 (cBGL1). By Northern blot analysis cBGL1 mRNA was expressed most abundantly in the liver, followed by the small intestine, colon, spleen, and kidney. When klotho and cBGL1 gene expression was examined in renal cell carcinoma tissues, both klotho and cBGL1 mRNA levels in tumors were lower than those in nontumor regions, suggesting that renal epithelial cells may lose klotho and cBGL1 gene expression during the course of malignant transformation. In conclusion, we describe the primary structure and gene expression of a novel protein related to klotho protein.

Klotho β-Glucosidase Gene expression Kidney Renal cell carcinoma

Copyright information

© Springer-Verlag 2000