Journal of Molecular Medicine

, Volume 90, Issue 8, pp 925–934

Chemopreventive effect of the non-psychotropic phytocannabinoid cannabidiol on experimental colon cancer

Authors

  • Gabriella Aviello
    • Department of Experimental Pharmacology, Endocannabinoid Research GroupUniversity of Naples Federico II
  • Barbara Romano
    • Department of Experimental Pharmacology, Endocannabinoid Research GroupUniversity of Naples Federico II
  • Francesca Borrelli
    • Department of Experimental Pharmacology, Endocannabinoid Research GroupUniversity of Naples Federico II
  • Raffaele Capasso
    • Department of Experimental Pharmacology, Endocannabinoid Research GroupUniversity of Naples Federico II
  • Laura Gallo
    • Institute of Biomolecular Chemistry, Endocannabinoid Research GroupConsiglio Nazionale delle Ricerche (CNR)
  • Fabiana Piscitelli
    • Institute of Biomolecular Chemistry, Endocannabinoid Research GroupConsiglio Nazionale delle Ricerche (CNR)
    • Institute of Biomolecular Chemistry, Endocannabinoid Research GroupConsiglio Nazionale delle Ricerche (CNR)
    • Institute of Biomolecular ChemistryCNR
    • Department of Experimental Pharmacology, Endocannabinoid Research GroupUniversity of Naples Federico II
    • Department of Experimental PharmacologyUniversity of Naples Federico II
Original Article

DOI: 10.1007/s00109-011-0856-x

Cite this article as:
Aviello, G., Romano, B., Borrelli, F. et al. J Mol Med (2012) 90: 925. doi:10.1007/s00109-011-0856-x

Abstract

Colon cancer affects millions of individuals in Western countries. Cannabidiol, a safe and non-psychotropic ingredient of Cannabis sativa, exerts pharmacological actions (antioxidant and intestinal antinflammatory) and mechanisms (inhibition of endocannabinoid enzymatic degradation) potentially beneficial for colon carcinogenesis. Thus, we investigated its possible chemopreventive effect in the model of colon cancer induced by azoxymethane (AOM) in mice. AOM treatment was associated with aberrant crypt foci (ACF, preneoplastic lesions), polyps, and tumour formation, up-regulation of phospho-Akt, iNOS and COX-2 and down-regulation of caspase-3. Cannabidiol-reduced ACF, polyps and tumours and counteracted AOM-induced phospho-Akt and caspase-3 changes. In colorectal carcinoma cell lines, cannabidiol protected DNA from oxidative damage, increased endocannabinoid levels and reduced cell proliferation in a CB1-, TRPV1- and PPARγ-antagonists sensitive manner. It is concluded that cannabidiol exerts chemopreventive effect in vivo and reduces cell proliferation through multiple mechanisms.

Keywords

Non-psychotropic cannabinoidColon cancerAdenocarcinoma cellsDNA damage

Copyright information

© Springer-Verlag 2012