Journal of Molecular Medicine

, Volume 90, Issue 2, pp 127–138

ZnT-1 protects HL-1 cells from simulated ischemia–reperfusion through activation of Ras–ERK signaling

  • Ofer Beharier
  • Shani Dror
  • Shiri Levy
  • Joy Kahn
  • Merav Mor
  • Sharon Etzion
  • Daniel Gitler
  • Amos Katz
  • Anthony J. Muslin
  • Arie Moran
  • Yoram Etzion
Original Article

DOI: 10.1007/s00109-011-0845-0

Cite this article as:
Beharier, O., Dror, S., Levy, S. et al. J Mol Med (2012) 90: 127. doi:10.1007/s00109-011-0845-0

Abstract

Activation of ERK signaling may promote cardioprotection from ischemia–reperfusion (I/R) injury. ZnT-1, a protein that confers resistance from zinc toxicity, was found to interact with Raf-1 kinase through its C-terminal domain, leading to downstream activation of ERK. In the present study, we evaluated the effects of ZnT-1 in cultured murine cardiomyocytes (HL-1 cells) that were exposed to simulated-I/R. Cellular injury was evaluated by lactate dehydrogenase (LDH) release and by staining for pro-apoptotic caspase activation. Overexpression of ZnT-1 markedly reduced LDH release and caspase activation following I/R. Knockdown of endogenous ZnT-1 augmented the I/R-induced release of LDH and increased caspase activation following I/R. Phospho-ERK levels were significantly increased following I/R in cells overexpressing ZnT-1, while knockdown of ZnT-1 reduced phospho-ERK levels. Pretreatment of cells with the MEK inhibitor PD98059 abolished the protective effect of ZnT-1 following I/R. Accordingly, a truncated form of ZnT-1 lacking the C-terminal domain failed to induce ERK activation and did not protect the cells from I/R injury. In contrast, expression of the C-terminal domain by itself was sufficient to induce ERK activation and I/R protection. Interestingly, the C-terminal of the ZnT-1 did not have protective effect against the toxicity of zinc. In the isolated rat heart, global ischemic injury rapidly increased the endogenous levels of ZnT-1. However, following reperfusion ZnT-1 levels were found to be decreased. Our findings indicate that ZnT-1 may have important role in the ischemic myocardium through its ability to interact with Raf-1 kinase.

Keywords

Cardiomyocyte survivalReperfusion injury salvage kinaseRaf-1 kinaseExtracellular signal-regulated kinase

Supplementary material

109_2011_845_MOESM1_ESM.doc (325 kb)
ESM 1(DOC 325 kb)

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Ofer Beharier
    • 1
    • 4
  • Shani Dror
    • 1
    • 4
  • Shiri Levy
    • 1
  • Joy Kahn
    • 1
  • Merav Mor
    • 1
    • 4
  • Sharon Etzion
    • 4
  • Daniel Gitler
    • 1
  • Amos Katz
    • 3
    • 4
  • Anthony J. Muslin
    • 2
  • Arie Moran
    • 1
  • Yoram Etzion
    • 5
  1. 1.Department of PhysiologyFaculty of Health Sciences, Ben-Gurion University of the NegevBeer-ShevaIsrael
  2. 2.Center for Cardiovascular Research, John Milliken Department of MedicineWashington University School of MedicineSt. LouisUSA
  3. 3.Department of CardiologyBarzilai Medical CenterAshkelonIsrael
  4. 4.Cardiac Arrhythmia Research LaboratorySoroka University Medical CenterBeer-ShevaIsrael
  5. 5.Cardiac Arrhythmia Research LaboratoryFaculty of Health sciences, Ben-Gurion University of the Negev & Soroka University Medical CenterBeer-ShevaIsrael