OSBPL10, a novel candidate gene for high triglyceride trait in dyslipidemic Finnish subjects, regulates cellular lipid metabolism
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- Perttilä, J., Merikanto, K., Naukkarinen, J. et al. J Mol Med (2009) 87: 825. doi:10.1007/s00109-009-0490-z
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Analysis of variants in three genes encoding oxysterol-binding protein (OSBP) homologues (OSBPL2, OSBPL9, OSBPL10) in Finnish families with familial low high-density lipoprotein (HDL) levels (N = 426) or familial combined hyperlipidemia (N = 684) revealed suggestive linkage of OSBPL10 single-nucleotide polymorphisms (SNPs) with extreme end high triglyceride (TG; >90th percentile) trait. Prompted by this initial finding, we carried out association analysis in a metabolic syndrome subcohort (Genmets) of Health2000 examination survey (N = 2,138), revealing association of multiple OSBPL10 SNPs with high serum TG levels (>95th percentile). To investigate whether OSBPL10 could be the gene underlying the observed linkage and association, we carried out functional experiments in the human hepatoma cell line Huh7. Silencing of OSBPL10 increased the incorporation of [3H]acetate into cholesterol and both [3H]acetate and [3H]oleate into triglycerides and enhanced the accumulation of secreted apolipoprotein B100 in growth medium, suggesting that the encoded protein ORP10 suppresses hepatic lipogenesis and very-low-density lipoprotein production. ORP10 was shown to associate dynamically with microtubules, consistent with its involvement in intracellular transport or organelle positioning. The data introduces OSBPL10 as a gene whose variation may contribute to high triglyceride levels in dyslipidemic Finnish subjects and provides evidence for ORP10 as a regulator of cellular lipid metabolism.
KeywordsCholesterolHigh-density lipoproteinMicrotubuleOxysterol-binding proteinSingle-nucleotide polymorphismTriglyceride
Families of proteins with homology to the carboxy-terminal ligand binding domain of oxysterol binding protein (OSBP) are suggested to function in various cellular processes such as lipid metabolism, intracellular lipid transport, membrane trafficking, and cell signaling [1, 2]. In humans, the gene family consists of 12 members, which are denoted OSBP-like genes (OSBPL), while the encoded proteins are called either OSBP-like (OSBPL) or OSBP-related proteins (ORP) [3, 4].
OSBP is the most extensively studied member of the mammalian ORP protein family. OSBP acts as a sterol sensor that integrates the cellular sterol status with sphingomyelin metabolism  and controls the dephosphorylation and hence the activity of extracellular signal-regulated kinases, ERK . We recently showed that OSBP overexpression in mouse liver impacts serum very-low-density lipoprotein triglyceride (TG) levels and hepatic TG synthesis through up-regulation of the expression and activity of sterol regulatory element binding protein 1c (SREBP-1c) , a major insulin-responsive regulator of hepatic and adipose tissue lipogenesis. Over-expression of human ORP1L in mouse macrophages resulted in impaired cholesterol efflux and increased size of atherosclerotic lesions in low-density lipoprotein (LDL)-receptor deficient mice . Furthermore, our latest data demonstrates a role of the OSBP homologue ORP8 as factor that suppresses, most likely via an indirect mechanism, the expression of ATP-binding cassette transporter A1 (ABCA1) , a key mediator of high-density lipoprotein (HDL) biogenesis. Moreover, ORP2, ORP4S, and ORP9 have been implicated in cellular sterol or neutral lipid metabolism [10–12], lending further support to the view that ORP family members play important roles in cellular lipid metabolism.
In the present study, we assessed whether allelic diversity of the OSBPL2, OSBPL9, or OSBPL10 genes contributes to extreme serum lipid levels in Finnish families ascertained for low HDL-cholesterol or familial combined hyperlipidemia (FCHL, common dyslipidemias associated with increased risk of premature coronary heart disease [13–16]. Variants of OSBPL10 displayed suggestive linkage with high triglyceride levels in the families, and association analysis in a metabolic syndrome study sample (N = 2,138) strengthened the notion that variation in OSBPL10 may influence serum TG levels in Finnish dyslipidemic subjects. RNA interference experiments in cultured human hepatoma cells suggested that the encoded protein, ORP10, acts as a regulator of cellular lipid biosyntheses and apolipoprotein B-100 (apoB100) secretion, lending functional support to the genetic findings.
Materials and methods
Dyslipidemic cohorts and genotyping strategy
Phenotypic characteristics of the low-HDL and FCHL study samples
45.30 ± 22.08
45.61 ± 20.5
45.47 ± 21.2
25.47 ± 4.25
24.76 ± 4.66
25.09 ± 4.5
5,40 ± 1.16
5,47 ± 1.07
5,44 ± 1.11
1.02 ± 0.3
1.35 ± 0.38
1.20 ± 0.38
1.59 ± 0.8
1.45 ± 0.79
1.51 ± 0.8
37.60 ± 20.44
37.91 ± 20.35
37.7 ± 20.4
25.56 ± 4.46
24.90 ± 5.29
25.22 ± 5.02
5.84 ± 1.43
5.85 ± 1.32
5.84 ± 1.37
1.15 ± 0.36
1.39 ± 0.41
1.27 ± 0.41
2.11 ± 1.88
1.59 ± 1.27
1.85 ± 1.6
The Genmets sample is subset of 2,212 individuals of the Health2000 study (http://www.terveys2000.fi/doc/methodologyrep.pdf) collected as metabolic syndrome cases and their matched controls. A total of 2,173 of these individuals have been genotyped on Illumina 610K arrays. In this study, 102 SNPs located in OSBPL10 or its flanking regions were analyzed. For the association analysis, individuals with the TG levels above the 95th population, sex, and age-specific percentile (>3.4 mmol/l) were coded as cases and the rest as controls. The same coding was done for the individuals with the high-density lipoprotein cholesterol values below the 5th percentile (<0.8 mmol/l). For these two dichotomous phenotypes, logistic regression was applied with age and sex as covariates (for details, see Electronic Supplementary Materials method descriptions).
The study materials were collected according to the Helsinki declaration, and the ethics committees of the participating centers approved the study design.
Generation of a rabbit antibody against ORP10
A cDNA fragment encoding amino acid residues 1–80 of the full-length ORP10 protein (NM_017784) was inserted in pGEX1λT (GE Healthcare, Uppsala, Sweden) for production of a glutathione-S-transferase fusion protein in Escherichia coli BL21. The protein was purified on Glutathione-Sepharose 4B (GE Healthcare) and used for subcutaneous immunization of New Zealand white rabbits by a standard procedure.
The human hepatoma cell line Huh7 was cultured in Eagle’s minimal essential medium with Earle’s salts (EMEM, Sigma-Aldrich, St. Louis, MO, USA), 20 mM Hepes, pH 7.4, 10% fetal bovine serum (FBS; Gibco/Invitrogen, Grand Island, NY, USA), 100 U/ml penicillin, and 100 μg/ml streptomycin. The pancreatic adenocarcinoma cell line PANC-1 was cultured in RPMI 1640 (BioWhittaker, Walkersville, MD, USA), 10% FBS, and the above antibiotics. Caco-2 cells were cultured in EMEM supplemented with 10% FBS, non-essential amino acids, and the above antibiotics.
Huh7 cells were transfected with ORP10-specific (siORP10.1, sense strand CCACAGCCUCAAUCUUGUAdTdT; siORP10.2, GAGAAUUUCCUGUGGAU-UAdTdT) or nontargeting control siRNA (siNT, sense strand UAGCGACUAAACACAUCAAdTdT) using INTERFERin (Polyplus, Illkirch, France) or HiPerFect (Qiagen, Valencia, CA, USA) according to the manufacturers’ instructions. After 48 h transfection, the cells were either subjected to total RNA isolation by using the RNeasy Mini kit (Qiagen) or used for analysis of cholesterol and triglyceride biosynthesis.
Analysis of cholesterol and triglyceride biosynthesis
Huh7 cells treated with ORP10-specific or nontargeting control siRNAs were labeled with [3H]acetic acid or [3H]oleic acid, and incorporation of the radioactive tracers into cholesterol or TG was carried out, as specified in Electronic Supplementary Materials method descriptions.
Assays for apoA-I and apoB100 secretion by Huh7 cells
Huh7 cells treated for 48 h with nontargeting or ORP10-specific siRNAs as specified above were washed with phosphate-buffered saline and transferred into serum-free culture medium. The medium and the cells were harvested at 6, 12, and 24 h. The apoA-I  and apoB100 (Mabtech, Nacka Strand, Sweden) concentrations in the medium were determined with specific sandwich enzyme-linked immunosorbent assay (ELISA) assays and normalized for total cell protein.
Total RNA was isolated from Huh7 cells treated with ORP10 or control siRNAs, and quantitative real-time polymerase chain reaction (PCR) analysis of specific mRNAs was carried out using SYBR-green (Applied Biosystems) as described in Electronic Supplementary Materials method descriptions. The primers used are specified in Electronic Supplementary Materials Table 2.
Assay for 3-hydroxy-3-methylglutaryl coenzyme A reductase activity
Huh7 cells treated with ORP10-specific or control siRNAs as specified above were harvested, and 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase activity in total membranes of the cells was assayed as detailed in Electronic Supplementary Materials method descriptions.
Analysis of the subcellular localization of ORP10
The human ORP10 open reading frame (NM_017784) was subcloned into the pEGFP-C (BD Biosciences Clontech, Palo Alto, CA, USA) or pcDNA4HisMax (Invitrogen) vectors for mammalian cell expression. Huh7, PANC-1, or Caco-2 cells cultured in normal serum-containing growth medium were transfected for 24 h using Lipofectamine 2000 (Invitrogen), fixed with paraformaldehyde and processed for immunofluorescence microscopy essentially as described previously . The immunofluorescence double stainings carried out, and pretreatments of the cells are described in Electronic Supplementary Materials method descriptions. Fluorescence recovery after photobleaching (FRAP) in Huh7 cells transfected with EGFP-ORP10 was carried out as detailed in Electronic Supplementary Materials method descriptions.
Single-nucleotide polymorphisms in OSBPL10 show suggestive linkage with serum triglyceride levels
Linkage and association results for the dichotomized HDL-cholesterol and TG traits in the low-HDL and FCHL families
<10th %a HDL
>90th % TG
<10th % HDL
>90th % TG
<10th % HDL
>90th % TG
We next studied whether the SNP alleles with the best evidence for linkage with the extreme end of TG concentration show correlation with the OSBPL10 mRNA expression level in fat biopsies withdrawn from 47 individuals belonging to the low-HDL and FCHL families, using transcriptome data acquired with Affymetrix U133 Plus 2.0 microarrays. No significant correlation was detected (data not shown), suggesting that rather than affecting the OSBPL10 mRNA levels, the SNPs may be in linkage disequilibrium (LD) with a yet unidentified functional variant.
Genmets study material: OSBPL10 SNPs associated with high TG (>95th percentile), p < 0.01
Effect all. frequency
ORP10 modulates hepatocellular lipogenesis
To investigate whether the elevation of [3H]cholesterol biosynthesis in Huh7 cells subjected to ORP10 silencing reflects a change in the activity of the rate-limiting enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, we determined in vitro the activity of this enzyme in membranes isolated from cells treated with ORP10-specific or control siRNAs. Incorporation of [14C]HMG-CoA into mevalonate was significantly (siORP10.1, 29%; siORP10.2, 21%) enhanced in cells subjected to ORP10 silencing as compared to those treated with the nontargeting control siRNA (Fig. 3c). Furthermore, to test if the effect of OSBPL10 silencing on TG biosynthesis occurs at the level of fatty acid biosynthesis or at the level of their activation or incorporation into TG, we assessed TG synthesis using [3H]oleic acid as the radioactive precursor. The results revealed enhancement in its incorporation into TG in cells subjected to ORP10 silencing, similar in its extent to the effect observed upon [3H]acetic acid labeling (Fig. 3d).
ORP10 localizes on microtubules
To investigate the dynamic parameters of EGFP-ORP10 microtubule association, transfected Huh7 cells were subjected to fluorescence recovery after photobleaching analysis using a confocal microscope set up for live cell analysis. After photobleaching the microtubule-associated EGFP-ORP10, fluorescence recovered to approximately 70% of the starting value during the 6-min analysis period (Fig. 6l; Electronic Supplementary Materials: Time lapse video). The recovery followed a kinetics which curve-fitting suggested to arise from two components. The t1/2 for the fast component was 4.5 ± 0.94 s, and that for the slow one was 64.3 ± 11.6 s.
In the present study, we investigated a putative function of OSBP-related proteins in the regulation of serum lipids and lipoproteins in humans. Genotyping of OSBPL gene SNPs in a dyslipidemic family material was initially used to evaluate the contribution of OSBPL genes to extreme lipid levels predisposing to cardiovascular disease, high triglycerides, and low HDL. Although recent genome-wide association studies [21–23] have initially identified common variants affecting lipid concentrations at the population level, data are also emerging for the impact of multiple rare variants influencing the extreme lipid levels . Here, we wanted to study such potential loci by testing the involvement of select OSBPL genes in carefully phenotyped Finnish families ascertained for dyslipidemias, low HDL, or FCHL. The FCHL study set revealed suggestive linkage of several SNPs in OSBPL10 to the high TG trait, and analysis of the combined material strengthened the evidence. Importantly, association analysis using an independent study sample consisting of subjects with metabolic syndrome and matched healthy controls provided substantial proof for association of OSBPL10 SNPs with the extreme end (>95th percentile) high-TG trait.
The genetic analyses suggest that variation within the chromosome 3p23 region containing OSBPL10 may contribute to the high TG levels in Finnish dyslipidemic subjects. The present data do not allow us to firmly conclude that the underlying gene is OSBPL10, but they prompted us to investigate in more detail the function of the encoded protein, ORP10, in cultured cell models. The metabolic studies in cultured cells subjected to ORP10 silencing identify the protein as a novel regulator of hepatocellular lipid metabolism and apoB100 secretion, consistent with the notion that OSBPL10 may be the gene underlying the genetic findings. The likelihood of this is further increased by the lack of other genes plausibly connected with lipid metabolism within the chromosome region (10 Mb interval).
Hepatoma cells subjected to ORP10 silencing displayed a significant increase in the incorporation of [3H]acetate into cholesterol and of [3H]acetate and [3H]oleic acid into TG, suggesting that the intrinsic function of ORP10 involves suppression of hepatocellular lipid biosyntheses. Consistent with this observation, the cells showed increased HMG-CoA reductase activity, demonstrating an effect already at an early stage of the pathway, on conversion of HMG-CoA to mevalonic acid. An independent, systematic esiRNA silencing screen of all ORP family members carried out in HeLa cells supported a role of ORP10 in the regulation of cholesterol biosynthesis (data not shown). Labeling with [3H]oleic acid suggested that the impact of ORP10 on TG biosynthesis occurs at the levels of fatty acid activation or acyl transfer to glycerol. Importantly, we also found that ORP10 silencing enhances the accumulation of apoB100 in the hepatoma cell growth medium. One can envision that ORP10 could affect the synthesis of TG and cholesterol as well as the assembly of TG-rich lipoproteins in the intestine and the liver, both tissues in which OSBPL10 mRNA is expressed abundantly .
ORP10 was found to distribute between a cytosolic localization and microtubules, a major apparatus determining the spatial distribution of organelles and forming tracks for intracellular transport events . FRAP analysis of live Huh7 cells with EGFP-ORP10 on microtubules revealed a recovery with two kinetic components. We find it likely that the fast component (t1/2 = 4.5 s) reflects the diffusion of EGFP-ORP10 in the cytosolic compartment, while the slow component (t1/2 = 64.3 s) could represent the actual microtubule association of the protein. The latter half-time is in the same range as the life-times determined for transport carriers moving along microtubules from the endoplasmic reticulum (ER) to the Golgi complex  and from the trans-Golgi network to the plasma membrane [27, 28]. The data are thus consistent with the notion that ORP10 could be involved in microtubule-dependent membrane trafficking or organelle motility.
Microtubule-dependent membrane trafficking is intimately connected with the control of lipid metabolism. Importantly, the membrane compartments that play key roles in the cellular metabolism of cholesterol and triglycerides, the ER and cytoplasmic lipid droplets, are connected to microtubules, which determine the spatial organization of the ER  and control the distribution and motility of lipid droplets [30, 31]. Pertinent to serum lipoproteins, also the Golgi complex playing a central role in lipoprotein secretion is organized by microtubules . One can therefore envision that a protein associated with microtubules, such as ORP10, is in the position to modulate cellular lipid metabolism and lipoprotein secretion.
We are grateful to Pipsa Kaipainen, Sari Nuutinen, Marja-Leena Piironen, Seija Puomilahti, and Pirjo Ranta for expert technical assistance. Dr. Markku Lehto is thanked for kindly providing the full-length ORP10 cDNA, Pekka Ellonen for help with the genotyping work, and Dr. Matti Jauhiainen for valuable comments on the manuscript. This study was supported by The Academy of Finland (grants 113013, 118720, and 121457 to V.M.O.), the European Union FP7 (LipidomicNet, agreement no. 202272), the Sigrid Juselius Foundation, the Finnish Foundation for Cardiovascular Research (M.-R.T., V.M.O.), the Magnus Ehrnrooth Foundation (V.M.O.), the Finnish Cultural Foundation (V.M.O.), and Helsinki University Central Hospital: A special governmental subsidy for health sciences research (M.-R.T., I.V.). Further support was provided by The Center of Excellence in Complex Disease Genetics (L.P., K.M., J.N.), The Research Program of Molecular Medicine, University of Helsinki, Biomedicum Foundation, and Biocentrum Helsinki Foundation (L.P., M.-R.T., K.M., J.N.).
Concerning this work, the authors have no conflicts of interest to declare.
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