Journal of Molecular Medicine

, Volume 87, Issue 1, pp 43–51

MicroRNA-128 inhibits glioma cells proliferation by targeting transcription factor E2F3a

Authors

  • Yu Zhang
    • The National Laboratory of Medical Molecular Biology, Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical College
    • Graduate School, Peking Union Medical CollegeTsinghua University
  • Tengfei Chao
    • The National Laboratory of Medical Molecular Biology, Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical College
    • Graduate School, Peking Union Medical CollegeTsinghua University
  • Ran Li
    • The National Laboratory of Medical Molecular Biology, Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical College
    • Graduate School, Peking Union Medical CollegeTsinghua University
  • Wei Liu
    • The National Laboratory of Medical Molecular Biology, Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical College
    • Graduate School, Peking Union Medical CollegeTsinghua University
  • Yang Chen
    • The National Laboratory of Medical Molecular Biology, Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical College
    • Graduate School, Peking Union Medical CollegeTsinghua University
  • Xingqi Yan
    • The National Laboratory of Medical Molecular Biology, Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical College
    • Graduate School, Peking Union Medical CollegeTsinghua University
  • Yanhua Gong
    • The National Laboratory of Medical Molecular Biology, Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical College
  • Bin Yin
    • The National Laboratory of Medical Molecular Biology, Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical College
  • Wei Liu
    • The Department of NeurosurgeryBeijing Tiantan Hospital
  • Boqing Qiang
    • The National Laboratory of Medical Molecular Biology, Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical College
  • Jizhong Zhao
    • The Department of NeurosurgeryBeijing Tiantan Hospital
    • The National Laboratory of Medical Molecular Biology, Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical College
    • The National Laboratory of Medical Molecular Biology, Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical College
Original Article

DOI: 10.1007/s00109-008-0403-6

Cite this article as:
Zhang, Y., Chao, T., Li, R. et al. J Mol Med (2009) 87: 43. doi:10.1007/s00109-008-0403-6

Abstract

MicroRNAs are ∼21nt single-stranded RNAs and function as regulators of gene expression. Previous studies have shown that microRNAs play crucial roles in tumorigenesis by targeting the mRNAs of oncogenes or tumor suppressors. Here we show that brain-enriched miR-128 is down-regulated in glioma tissues and cell lines when compared to normal brain tissues. Overexpression of miR-128 in glioma cells inhibited cell proliferation. A bioinformatics search revealed a conserved target site within the 3′untranslated region (UTR) of E2F3a, a transcription factor that regulates cell cycle progression. The protein levels of E2F3a in gliomas and normal brain tissues were negatively correlated to the expression levels of miR-128 in these tissues. Overexpression of miR-128 suppressed a luciferase-reporter containing the E2F3a-3′UTR and reduced the level of E2F3a protein in T98G cells. Moreover, knocking down of E2F3a had similar effect as overexpression of miR-128, and overexpression of E2F3a can partly rescue the proliferation inhibition caused by miR-128. Taken together, our study demonstrates that miR-128 can inhibit proliferation of glioma cells through one of its targets, E2F3a.

Keywords

miR-128E2F3aGliomaProliferation

Supplementary material

109_2008_403_MOESM1_ESM.doc (124 kb)
ESM(DOC 124 KB)

Copyright information

© Springer-Verlag 2008