Journal of Molecular Medicine

, Volume 86, Issue 4, pp 391–400

Diabetes-induced alteration of F4/80+ macrophages: a study in mice with streptozotocin-induced diabetes for a long term

Authors

  • Haixia Ma
    • Transplantation Biology Research Division, State Key Laboratory of Biomembrane and Membrane BiotechnologyInstitute of Zoology, Chinese Academy of Sciences
    • Graduate SchoolChinese Academy of Sciences
    • China–U.S. Jointed Research Center for Life SciencesChinese Academy of Sciences
  • Guangwei Liu
    • Transplantation Biology Research Division, State Key Laboratory of Biomembrane and Membrane BiotechnologyInstitute of Zoology, Chinese Academy of Sciences
    • China–U.S. Jointed Research Center for Life SciencesChinese Academy of Sciences
  • Wenjun Ding
    • Graduate SchoolChinese Academy of Sciences
  • You Wu
    • Transplantation Biology Research Division, State Key Laboratory of Biomembrane and Membrane BiotechnologyInstitute of Zoology, Chinese Academy of Sciences
  • Lu Cai
    • Department Radiation OncologyThe University of Louisville
    • Department of MedicineThe University of Louisville
    • Transplantation Biology Research Division, State Key Laboratory of Biomembrane and Membrane BiotechnologyInstitute of Zoology, Chinese Academy of Sciences
    • China–U.S. Jointed Research Center for Life SciencesChinese Academy of Sciences
Original Article

DOI: 10.1007/s00109-008-0304-8

Cite this article as:
Ma, H., Liu, G., Ding, W. et al. J Mol Med (2008) 86: 391. doi:10.1007/s00109-008-0304-8

Abstract

Macrophages as an early stage of immune responses form a bridge between innate and acquired immunity and shape the adaptive immune response. The immunoregulatory functions of macrophages in hosts with a prolonged exposure to a diabetic milieu remain to be determined. The levels, phenotype, and immunity including antigen-presenting ability, phagocytosis and immunogenicity of F4/80+ splenic macrophages (SPMs), and peritoneal exudates macrophages (PEMs) were detected in age-matched control mice and mice with streptozotocin (STZ)-induced diabetes for 16 weeks. The numbers of F4/80+ SPMs and PEMs significantly decreased in STZ-induced diabetic mice, compared with age-matched non-diabetic mice (control) at 16 weeks after diabetes induction. Functional analysis showed that F4/80+ SPMs and PEMs in STZ-induced diabetic mice exhibit significantly lower immunogenicity and nonopsonic phagocytosis to allogeneic T cells than those of control mice both in vitro and in vivo. Coincidently, the antigen-presenting capacity of F4/80+ PEMs, but not F4/80+ SPMs, in mice with STZ-induced diabetes for 16 or more weeks is also significantly lower than that of control mice. Our results showed that total cell number and immune function of F4/80+ macrophages were significantly defective in mice with a prolonged exposure to a diabetic milieu, which may be a mechanism responsible for the increased macrophage-related complications in diabetic patients such as the high prevalence of infection and cardiovascular mortality.

Keywords

Monocytes/macrophagesDiabetesAutoimmuneImmunodeficiencyDiabetic complications

Abbreviations

APCs

antigen-presenting cells

DCs

dendritic cells

DTH

delayed-type hypersensitivity

FCM

flow cytometry

IFN-γ

interferon-γ

IL-2

interleukin-2

MFI

median fluorescence intensity

MLR

mixed leukocyte reactions

PEMs

peritoneal exudate macrophages

SPMs

splenic macrophages

Copyright information

© Springer-Verlag 2008