Journal of Molecular Medicine

, Volume 83, Issue 5, pp 362–376

Gene expression profiling: cell cycle deregulation and aneuploidy do not cause breast cancer formation in WAP-SVT/t transgenic animals

  • Andreas Klein
  • Eva Guhl
  • Raphael Zollinger
  • Yin-Jeh Tzeng
  • Ralf Wessel
  • Michael Hummel
  • Monika Graessmann
  • Adolf Graessmann
Original Article

DOI: 10.1007/s00109-004-0625-1

Cite this article as:
Klein, A., Guhl, E., Zollinger, R. et al. J Mol Med (2005) 83: 362. doi:10.1007/s00109-004-0625-1

Abstract

Microarray studies revealed that as a first hit the SV40 T/t antigen causes deregulation of 462 genes in mammary gland cells (ME cells) of WAP-SVT/t transgenic animals. The majority of deregulated genes are cell proliferation specific and Rb-E2F dependent, causing ME cell proliferation and gland hyperplasia but not breast cancer formation. In the breast tumor cells a further 207 genes are differentially expressed, most of them belonging to the cell communication category. In tissue culture breast tumor cells frequently switch off WAP-SVT/t transgene expression and regain the morphology and growth characteristics of normal ME cells, although the tumor-revertant cells are aneuploid and only 114 genes regain the expression level of normal ME cells. The profile of retransformants shows that only 38 deregulated genes are tumor-specific, and that none of them is considered to be a typical breast cancer gene.

Keywords

Breast cancerMammary gland epithelial cellsSV40 T/t antigenTumorigenesisWAP-SVT/t transgene

Abbreviations

CA

Correspondence analysis

DMEM

Dulcecco’s modified Eagle medium

IRF

Interferon regulatory factor

ME

Mammary gland epithelial

OAS

2′-5′-Oligoadenylate synthetase family members

PBS

Phosphate-buffered saline

SV40

Simian virus 40

WAP

Whey acidic milk protein

Supplementary material

Supplement 1 462 differentially expressed genes from normal to transgenic animals (first hit)

Supplement_1.pdf (93 kb)
(PDF 93 KB)

Supplement 2 207 differentially expressed genes from transgenic to tumor animals (second hit)

Supplement_2.pdf (58 kb)
(PDF 58 KB)

Supplement 3 114 differentially expressed genes from breast tumor to Revertant-ME-B cells

Supplement_3.pdf (94 kb)
(PDF 95 KB)

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • Andreas Klein
    • 1
  • Eva Guhl
    • 1
  • Raphael Zollinger
    • 2
  • Yin-Jeh Tzeng
    • 3
  • Ralf Wessel
    • 1
  • Michael Hummel
    • 2
  • Monika Graessmann
    • 1
  • Adolf Graessmann
    • 1
  1. 1.Institut für Molekularbiologie und Bioinformatik, Charité Hospital, Campus Benjamin FranklinUniversitätsmedizin BerlinBerlinGermany
  2. 2.Institut für Pathologie, Charité Hospital, Campus Benjamin FranklinUniversitätsmedizin BerlinBerlinGermany
  3. 3.Graduate Institute of Molecular and Cell BiologyTzu-Chi UniversityHualienTaiwan