Journal of Molecular Medicine

, Volume 81, Issue 7, pp 428–434

Allele-specific differential expression of a common adiponectin gene polymorphism related to obesity

Authors

  • Wei-Shiung Yang
    • Division of Endocrinology and Metabolism, Departments of Internal MedicineNational Taiwan University Hospital
    • Graduate Institute of Clinical MedicineNational Taiwan University Medical College
  • Pei-Ling Tsou
    • Department of Clinical PathologyNational Taiwan University Hospital
  • Wei-Jei Lee
    • Department of SurgeryEn-Chu-Kong Hospital
  • Da-Lun Tseng
    • Graduate Institute of Clinical MedicineNational Taiwan University Medical College
  • Chi-Ling Chen
    • Institute of EpidemiologyNational Taiwan University
  • Chi-Chung Peng
    • Division of Endocrinology and Metabolism, Departments of Internal MedicineNational Taiwan University Hospital
  • Kuan-Ching Lee
    • Division of Endocrinology and Metabolism, Departments of Internal MedicineNational Taiwan University Hospital
  • Mei-Ju Chen
    • Division of Endocrinology and Metabolism, Departments of Internal MedicineNational Taiwan University Hospital
  • Chang-Jen Huang
    • Institute of Biological ChemistryAcademia Sinica
  • Tong-Yuan Tai
    • Division of Endocrinology and Metabolism, Departments of Internal MedicineNational Taiwan University Hospital
    • Division of Endocrinology and Metabolism, Departments of Internal MedicineNational Taiwan University Hospital
    • Graduate Institute of Clinical MedicineNational Taiwan University Medical College
Original Article

DOI: 10.1007/s00109-002-0409-4

Cite this article as:
Yang, W., Tsou, P., Lee, W. et al. J Mol Med (2003) 81: 428. doi:10.1007/s00109-002-0409-4

Abstract

Adiponectin gene polymorphisms have recently been reported to be associated with obesity, insulin sensitivity, and the risk of type 2 diabetes. We examined a T94G polymorphism of the adiponectin gene in 245 ostensibly normal nondiabetic subjects. The G allele frequency was lower among subjects with higher BMI (≥27) than in those with lower BMI. BMI was inversely correlated with the dose of G allele. Multivariate linear regression analyses showed that the adiponectin genotypes were significantly related to BMI after adjusting for age and gender. The dose of the G allele was associated with a reduction of approximately 1.12 kg/m2 in BMI. We further found that the relative mRNA levels of G allele were consistently higher than those of T allele in the omental adipose tissue from 21 heterozygous subjects. Finally, we observed that the expression levels of adiponectin affected insulin-stimulated glucose uptake in differentiated 3T3-L1 adipocytes. In conclusion, the allele-specific differential expression of this common polymorphism could be responsible for its biological effects observed in this and the other studies.

Keywords

ObesityInsulin resistanceAdiponectinPolymorphismmRNA

Abbreviations

BMI

Body mass index

SNP

Single nucleotide polymorphism

Copyright information

© Springer-Verlag 2003