Medicinal Chemistry Research

, Volume 24, Issue 2, pp 459–467

Rapid determination of lenalidomide in rat plasma by an ultra performance liquid chromatography/tandem mass spectrometric method

Original Research

DOI: 10.1007/s00044-014-1106-x

Cite this article as:
Pocha, K. & Devala Rao, G. Med Chem Res (2015) 24: 459. doi:10.1007/s00044-014-1106-x
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Abstract

A new, rapid, and specific ultra performance liquid chromatographic assay with mass spectrometric detection has been developed and validated for the quantitative determination of the antiangiogenic agent lenalidomide in rat plasma using thalidomide as an internal standard with electro spray ionization in multiple-reaction monitoring mode. The analyte and internal standard were extracted by liquid–liquid extraction using methyl-tert-butyl ether solvent. Chromatography was performed isocratically on Acquity BEH C18 column using a mobile phase consisting of a mixture of buffer, acetonitrile, and formic acid in ratios of 30:70:0.1 (v/v/v). The method had a chromatographic run time of 1.2 min. The calibration curve was fit to a linear response concentration data over a range of 0.06–60.00 μM using a weighting factor of 1/×2. The intra- and inter-day precision and accuracy results were well within the acceptable limits. The validated method was successfully applied for routine analysis of the pharmacokinetics of lenalidomide.

Keywords

Lenalidomide Ultra performance liquid chromatography Tandem mass spectrometry Low sample volume Pharmacokinetics High throughput analysis 

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.KVSR Siddhartha College of Pharmaceutical SciencesVijayawadaIndia