Medicinal Chemistry Research

, Volume 19, Issue 8, pp 948–961

Inhibitory effect of Gymnema Montanum leaves on α-glucosidase activity and α-amylase activity and their relationship with polyphenolic content

  • Kunga Mohan Ramkumar
  • Balsamy Thayumanavan
  • Thayumanavan Palvannan
  • Palanisamy Rajaguru
Original Research

DOI: 10.1007/s00044-009-9241-5

Cite this article as:
Ramkumar, K.M., Thayumanavan, B., Palvannan, T. et al. Med Chem Res (2010) 19: 948. doi:10.1007/s00044-009-9241-5

Abstract

The present study was attempted to investigate the effect of G. montanum leaf extract on inhibition of α-glucosidase and α-amylase activity. The ethanol extract of G. montanum (GLEt) at various concentrations (1–10 μg/ml) was tested for its inhibition pattern against α-glucosidase and α-amylase activity in vitro and compared with the commercially available α-glucosidase inhibitor, acarbose. The GLEt showed competitive inhibition against yeast α-glucosidase and noncompetitive inhibition against salivary α-amylase in a concentration-dependent manner. Further, we investigated the effect of extract on levels of blood glucose and plasma insulin in neonatal streptozotocin-induced type 2 diabetic rats. Long-term administration (12 weeks) of the GLEt effectively reduced the blood glucose level and also increased the insulin level. In a preliminary phytochemical analysis, G. montanum leaves were rich in phenolic composition and found to be positively correlated with the inhibitory effect of α-glucosidase and α-amylase activity. These results suggest that the GLEt might exert its antidiabetic effect by suppressing carbohydrate absorption from the intestine and thereby reducing hyperglycemia.

Keywords

α-Glucosidase inhibitorα-Amylase inhibitorGymnema montanumType 2 diabetesnSTZ ratsMedicinal plants

Copyright information

© Birkhäuser Boston 2009

Authors and Affiliations

  • Kunga Mohan Ramkumar
    • 1
  • Balsamy Thayumanavan
    • 2
  • Thayumanavan Palvannan
    • 3
  • Palanisamy Rajaguru
    • 1
  1. 1.Department of BiotechnologyAnna University TiruchirappalliTiruchirappalliIndia
  2. 2.Department of Biochemistry, Centre for Plant Molecular BiologyTamilnadu Agricultural UniversityCoimbatoreIndia
  3. 3.Department of BiochemistryPeriyar UniversitySalemIndia