Medicinal Chemistry Research

, Volume 19, Issue 5, pp 448–461

Antioxidant activity of alcoholic extract of Agrimonia pilosa Ledeb

  • Chunhuan He
  • Xiaowen Ji
  • Yingming Pan
  • Hengshan Wang
  • Kai Wang
  • Min Liang
  • Lizhu Yang
Original Research

DOI: 10.1007/s00044-009-9201-0

Cite this article as:
He, C., Ji, X., Pan, Y. et al. Med Chem Res (2010) 19: 448. doi:10.1007/s00044-009-9201-0

Abstract

Ultrasonically assisted extraction and Soxhlet extraction using 95% ethanol were performed to obtain ultrasonically assisted extract of Agrimonia pilosa (UEA) and Soxhlet extract of Agrimonia pilosa (SEA). The effects of the different extracting methods on the total phenolic (TP) and total flavonoid (TF) content of the extracts from Agrimonia pilosa were investigated. The findings showed that UEA contained higher TP and TF content than SEA. To understand better the effect of ultrasonic extraction, the antioxidant activities of UEA and SEA were evaluated with different antioxidant testing systems in vitro to determine reducing power, total antioxidant capacity, and free radical scavenging assays, including 2,2′-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals. Both SEA and UEA exhibited satisfied antioxidant activity superior to that of synthetic antioxidant butylated hydroxytoluene (BHT). The antioxidant activity of the tested subjects was in the order of UEA > SEA > BHT. Their high phenolic and flavonoid contents could be responsible for their antioxidant activity and pharmacologic actions.

Keywords

ABTSAgrimonia pilosaAntioxidant activityTotal phenolicTotal flavonoidsUltrasonic extraction

Copyright information

© Birkhäuser Boston 2009

Authors and Affiliations

  • Chunhuan He
    • 1
  • Xiaowen Ji
    • 1
  • Yingming Pan
    • 1
  • Hengshan Wang
    • 1
  • Kai Wang
    • 1
  • Min Liang
    • 1
  • Lizhu Yang
    • 1
  1. 1.Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Chemical EngineeringGuangxi Normal UniversityGuilinPeople’s Republic of China