Insectes Sociaux

, Volume 50, Issue 2, pp 199–200

Identification of polygyne and monogyne fire ant colonies (Solenopsis invicta) by multiplex PCR of Gp-9 alleles

  • S. M. Valles
  • S. D. Porter
Short communication

DOI: 10.1007/s00040-003-0662-8

Cite this article as:
Valles, S. & Porter, S. Insectes soc. (2003) 50: 199. doi:10.1007/s00040-003-0662-8


Oligonucleotide primers were designed to discriminate between the Gp-9B and Gp-9b alleles found in monogyne and polygyne colonies of fire ant, Solenopsis invicta Buren. Primers specific for the Gp-9B allele produced a 517 bp amplicon and primers specific for Gp-9b allele produced a 423 bp amplicon. When both sets of primers were multiplexed, homozygous monogyne ants produced a single 517 bp amplicon (specific for Gp-9B), whereas heterozygous polygyne ants produced one 517 bp amplicon and one 423 bp amplicon (specific for Gp-9B and Gp-9b, respectively) which allowed the Gp-9 alleles to be discerned in a single reaction. This method was tested on ants from 20 monogyne colonies and 20 polygyne colonies and was 100% accurate in discriminating the two forms.

Key words: Monogyne, polygyne, Solenopsis invicta, Gp-9.

Copyright information

© Birkhäuser Verlag, 2003

Authors and Affiliations

  • S. M. Valles
    • 1
  • S. D. Porter
    • 1
  1. 1.USDA-ARS Center for Medical, Agricultural and Veterinary Entomology, 1600 SW 23nd Drive, Gainesville, Florida 32604, USA, e-mail: svalles@gainesville.usda.ufl.eduUS