Identification of polygyne and monogyne fire ant colonies (Solenopsis invicta) by multiplex PCR of Gp-9 alleles
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- Valles, S. & Porter, S. Insectes soc. (2003) 50: 199. doi:10.1007/s00040-003-0662-8
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Oligonucleotide primers were designed to discriminate between the Gp-9B and Gp-9b alleles found in monogyne and polygyne colonies of fire ant, Solenopsis invicta Buren. Primers specific for the Gp-9B allele produced a 517 bp amplicon and primers specific for Gp-9b allele produced a 423 bp amplicon. When both sets of primers were multiplexed, homozygous monogyne ants produced a single 517 bp amplicon (specific for Gp-9B), whereas heterozygous polygyne ants produced one 517 bp amplicon and one 423 bp amplicon (specific for Gp-9B and Gp-9b, respectively) which allowed the Gp-9 alleles to be discerned in a single reaction. This method was tested on ants from 20 monogyne colonies and 20 polygyne colonies and was 100% accurate in discriminating the two forms.