Cellular and Molecular Life Sciences CMLS

, Volume 60, Issue 3, pp 518–525

The role of interleukin-17 in inducible nitric oxide synthase-mediated nitric oxide production in endothelial cells

  • Dj. Miljkovic
  • I. Cvetkovic
  • O. Vuckovic
  • S. Stosic-Grujicic
  • M. Mostarica Stojkovic
  • V. Trajkovic
Research Article

DOI: 10.1007/s000180300043

Cite this article as:
Miljkovic, D., Cvetkovic, I., Vuckovic, O. et al. CMLS, Cell. Mol. Life Sci. (2003) 60: 518. doi:10.1007/s000180300043

Abstract.

The effect of interleukin (IL)-17 on the activation of inducible nitric oxide (NO) synthase (iNOS) and subsequent production of NO was investigated. IL-17 induced NO production in both mouse and rat endothelial cells in a dose- and time-dependent manner. This was paralleled by the induction of mRNA for iNOS, which was markedly down-regulated by specific antagonists of protein tyrosine kinase, p38 MAP kinase or iNOS transcription factor NF-κB. The expression of iNOS transcription factor IRF-1 was also induced by IL-17 and blocked by all three inhibitors, suggesting that the induction of iNOS by IL-17 might be partly exerted through IRF-1 activation. Neutralization with the specific antibody showed that endogenous IL-17 is involved in T cell-mediated NO production in endothelial cells and NO-dependent suppression of T cell growth. These data indicate that IL-17-triggered iNOS activation in endothelial cells might participate in regulation of the T cell-dependent inflammatory response.

Key words. Endothelial cell; interleukin-17; nitric oxide; iNOS; IRF-1. 

Copyright information

© Birkhäuser Verlag, 2003

Authors and Affiliations

  • Dj. Miljkovic
    • 1
  • I. Cvetkovic
    • 1
  • O. Vuckovic
    • 2
  • S. Stosic-Grujicic
    • 1
  • M. Mostarica Stojkovic
    • 2
  • V. Trajkovic
    • 2
  1. 1.Institute for Biological Research 'Sinisa Stankovic', 29 Novembra 142, 11060 Belgrade (Yugoslavia), Fax: +381 11 657 258, e-mail: georgije.zw@yahoo.comYU
  2. 2.Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Belgrade (Yugoslavia)YU