Cellular and Molecular Life Sciences CMLS

, Volume 60, Issue 2, pp 401–412

Novel complexes of cyclin-dependent kinases and a cyclin-like protein from Arabidopsis thaliana with a function unrelated to cell division

  • R. M. Barrôco
  • L. De Veylder
  • Z. Magyar
  • G. Engler
  • D. Inzé
  • V. Mironov
Research Article

DOI: 10.1007/s000180300033

Cite this article as:
Barrôco, R., De Veylder, L., Magyar, Z. et al. CMLS, Cell. Mol. Life Sci. (2003) 60: 401. doi:10.1007/s000180300033
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Abstract.

Although the majority of cyclin-dependent kinases (CDKs) play a key role in cell cycle progression, recent evidence has shown that CDKs are also implicated in transcription regulation. Here, we describe two Arabidopsis CDKs designated Arath;CDKC;1 and Arath; CDKC;2. These CDKs share a PITAIRE signature in the cyclin-binding domain and the structural characteristics of mammalian CDK9. Yeast two-hybrid screens and immunoprecipitation assays identified CDKC-interacting proteins with homology to the animal cyclin T/cyclin K group. We suggest that these Arabidopsis CDKCs may be part of a kinase complex similar to the animal positive transcription elongation factor b, whose activity is essential for transcription control. Expression studies showed that Arath; CDKC transcripts are mainly confined to epidermal tissues and are most abundant in flower tissues. No expression was detected in actively dividing Arabidopsis tissues, suggesting a role for the CDKC proteins in differentiated cells.

Key words. Arabidopsis thaliana; cell cycle; cyclin-dependent kinase (CDK); cyclin T; transcription control; two-hybrid interaction. 

Copyright information

© Birkhäuser Verlag, 2003

Authors and Affiliations

  • R. M. Barrôco
    • 1
  • L. De Veylder
    • 1
  • Z. Magyar
    • 1
  • G. Engler
    • 3
  • D. Inzé
    • 1
  • V. Mironov
    • 1
  1. 1.Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB), Ghent University, K.L. Ledeganckstraat 35, 9000 Gent (Belgium), Fax +32 9 2645349, e-mail: dirk.inze@gengenp.rug.ac.beBE
  2. 2.Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, 6726 Szeged (Hungary)HU
  3. 3.Laboratoire Associé de l'Institut National de la Recherche Agronomique (France)FR
  4. 4.Universiteit Gent, 9000 Gent (Belgium)BE

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