Cellular and Molecular Life Sciences CMLS

, Volume 57, Issue 5, pp 828–833

Novel insecticidal toxins from nematode-symbiotic bacteria

Authors

  • R. H. ffrench-Constant*
    • Department of Biology and Biochemistry, South Building, University of Bath, Bath BA2 7AY (United Kingdom), Fax +44 1225 826779, e-mail: bssrfc@bath.ac.uk
  • D. J. Bowen
    • Department of Entomology, University of Wisconsin-Madison, Madison (Wisconsin 53706, USA), e-mail: djbowen@facstaff.wisc.edu

DOI: 10.1007/s000180050044

Cite this article as:
ffrench-Constant*, R. & Bowen, D. CMLS, Cell. Mol. Life Sci. (2000) 57: 828. doi:10.1007/s000180050044

Abstract.

The current strategy of using transgenic crops expressing insecticidal protein toxins is placing increasing emphasis on the discovery of novel toxins, beyond those already derived from the bacterium Bacillus thuringiensis. Here we review the cloning of four insecticidal toxin complex (tc) encoding genes from a different bacterium Photorhabdus luminescens and of similar gene sequences from Xenorhabdus nematophilus. Both these bacteria occupy the gut of entomopathogenic nematodes and are released into the insect upon invasion by the nematode. In the insect the bacteria presumably secrete these insecticidal toxins, as well as a range of other antimicrobials, to establish the insect cadaver as a monocultural breeding ground for both bacteria and nematodes. In this review, the protein biochemistry and structure of the tc encoding loci are discussed in relation to their observed toxicity and histopathology. These toxins may prove useful as alternatives to those derived from B. thuringiensis for deployment in insect-resistant transgenic plants.

Key words. Insecticidal protein toxins; Bacillus thuringiensis; Photorhabdus luminescens; Xenorhabdus nematophilus.

Copyright information

© Birkhäuser Verlag Basel, 2000