Cellular and Molecular Life Sciences CMLS

, Volume 56, Issue 9, pp 843–856

Glycine: a new anti-inflammatory immunonutrient

  • M. D. Wheeler
  • K. Ikejema
  • N. Enomoto
  • R. F. Stacklewitz
  • V. Seabra
  • Z. Zhong
  • M. Yin
  • P. Schemmer
  • M. L. Rose
  • I. Rusyn
  • B. Bradford
  • R. G. Thurman

DOI: 10.1007/s000180050030

Cite this article as:
Wheeler, M., Ikejema, K., Enomoto, N. et al. CMLS, Cell. Mol. Life Sci. (1999) 56: 843. doi:10.1007/s000180050030

Abstract.

The mechanism of the immunosuppressive effects of glycine and its pathophysiological applications are discussed in this review. Glycine has been well characterized in spinal cord as an inhibitory neurotransmitter which activates a glycine-gated chloride channel (GlyR) expressed in postsynaptic membranes. Activation of the channel allows the influx of chloride, preventing depolarization of the plasma membrane and the potentiation of excitatory signals along the axon. Glycine has recently been shown to have similar inhibitory effects on several white blood cells, including hepatic and alveolar macrophages, neutrophils, and lymphocytes. Pharmacological analysis using a GlyR antagonist strychnine, chloride-free buffer, and radiolabeled chloride has provided convincing evidence to support the hypothesis that many white blood cells contain a glycine-gated chloride channel with properties similar to the spinal cord GlyR. Molecular analysis using reverse transcription-polymerase chain reaction and Western blotting has identified the mRNA and protein for the β subunit of the GlyR in total RNA and purified membrane protein from rat Kupffer cells. Dietary glycine is protective in rat models against endotoxemia, liver ischemia-reperfusion, and liver transplantation, most likely by inactivating the Kupffer cell via this newly identified glycine-gated chloride channel. Glycine also prevents the growth of B16 melanomas cell in vivo. Moreover, dietary glycine is protective in the kidney against cyclosporin A toxicity and ischemia-reperfusion injury. Glycine may be useful clinically for the treatment of sepsis, adult respiratory distress syndrome, arthritis, and other diseases with an inflammatory component.

Key words. Glycine; immunoregulation; anti-inflammatory; glycine receptor. 

Copyright information

© Birkhäuser Verlag Basel, 1998

Authors and Affiliations

  • M. D. Wheeler
    • 1
  • K. Ikejema
    • 1
  • N. Enomoto
    • 1
  • R. F. Stacklewitz
    • 1
  • V. Seabra
    • 1
  • Z. Zhong
    • 1
  • M. Yin
    • 1
  • P. Schemmer
    • 1
  • M. L. Rose
    • 1
  • I. Rusyn
    • 1
  • B. Bradford
    • 1
  • R. G. Thurman
    • 1
  1. 1.Department of Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill (North Carolina, USA)US

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