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Microscale imaging of cilia-driven fluid flow

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Abstract

Cilia-driven fluid flow is important for multiple processes in the body, including respiratory mucus clearance, gamete transport in the oviduct, right–left patterning in the embryonic node, and cerebrospinal fluid circulation. Multiple imaging techniques have been applied toward quantifying ciliary flow. Here, we review common velocimetry methods of quantifying fluid flow. We then discuss four important optical modalities, including light microscopy, epifluorescence, confocal microscopy, and optical coherence tomography, that have been used to investigate cilia-driven flow.

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Acknowledgments

We thank Ute Gamm for her useful feedback. This work was supported by a March of Dimes Basil O’Connor Starter Scholar Research Award and NIH 1R01HL118419-01. BKH also was supported by NIH MSTP TG T32GM07205.

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Authors and Affiliations

  1. Department of Biomedical Engineering, Yale University, New Haven, USA

    Brendan K. Huang & Michael A. Choma

  2. Department of Diagnostic Radiology, Yale School of Medicine, New Haven, USA

    Michael A. Choma

  3. Department of Pediatrics, Yale School of Medicine, New Haven, USA

    Michael A. Choma

  4. Department of Applied Physics, Yale University, New Haven, USA

    Michael A. Choma

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Huang, B.K., Choma, M.A. Microscale imaging of cilia-driven fluid flow. Cell. Mol. Life Sci. 72, 1095–1113 (2015). https://doi.org/10.1007/s00018-014-1784-z

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