Abstract
Fungal disease is an increasing problem in both agriculture and human health. Treatment of human fungal disease involves the use of chemical fungicides, which generally target the integrity of the fungal plasma membrane or cell wall. Chemical fungicides used for the treatment of plant disease, have more diverse mechanisms of action including inhibition of sterol biosynthesis, microtubule assembly and the mitochondrial respiratory chain. However, these treatments have limitations, including toxicity and the emergence of resistance. This has led to increased interest in the use of antimicrobial peptides for the treatment of fungal disease in both plants and humans. Antimicrobial peptides are a diverse group of molecules with differing mechanisms of action, many of which remain poorly understood. Furthermore, it is becoming increasingly apparent that stress response pathways are involved in the tolerance of fungi to both chemical fungicides and antimicrobial peptides. These signalling pathways such as the cell wall integrity and high-osmolarity glycerol pathway are triggered by stimuli, such as cell wall instability, changes in osmolarity and production of reactive oxygen species. Here we review stress signalling induced by treatment of fungi with chemical fungicides and antifungal peptides. Study of these pathways gives insight into how these molecules exert their antifungal effect and also into the mechanisms used by fungi to tolerate sub-lethal treatment by these molecules. Inactivation of stress response pathways represents a potential method of increasing the efficacy of antifungal molecules.
Similar content being viewed by others
References
Jenssen H, Hamill P, Hancock RE (2006) Peptide antimicrobial agents. Clin Microbiol Rev 19(3):491–511
Hancock REW, Lehrer R (1998) Cationic peptides: a new source of antibiotics. Trends Biotechnol 16(2):82–88
Zasloff M (2002) Antimicrobial peptides of multicellular organisms. Nature 415(6870):389–395
Oren Z, Shai Y (1998) Mode of action of linear amphipathic α-helical antimicrobial peptides. Pept Sci 47(6):451–463
Nicolas P (2009) Multifunctional host defense peptides: intracellular-targeting antimicrobial peptides. FEBS J 276(22):6483–6496
van der Weerden NL, Bleackley MR, Anderson MA (2013) Properties and mechanisms of action of naturally occurring antifungal peptides. Cell Mol Life Sci 70(19):3545–3570
Theis T, Stahl U (2004) Antifungal proteins: targets, mechanisms and prospective applications. Cell Mol Life Sci 61(4):437–455
Cudic M, Otvos L Jr (2002) Intracellular targets of antibacterial peptides. Curr Drug Targets 3(2):101–106
Nikolaou E, Agrafioti I, Stumpf M, Quinn J, Stansfield I, Brown A (2009) Phylogenetic diversity of stress signalling pathways in fungi. BMC Evol Biol 9(1):44
Smith DA, Morgan BA, Quinn J (2010) Stress signalling to fungal stress-activated protein kinase pathways. FEMS Microbiol Lett 306(1):1–8
Alonso-Monge R, Román E, Arana DM, Pla J, Nombela C (2009) Fungi sensing environmental stress. Clin Microbiol Infect 15:17–19
Monge RA, Román E, Nombela C, Pla J (2006) The MAP kinase signal transduction network in Candida albicans. Microbiol 152(4):905–912
Xu J-R (2000) MAP kinases in fungal pathogens. Fungal Genet Biol 31(3):137–152
Gustin M, Albertyn J, Alexander M, Davenport K (1998) MAP kinase pathways in the yeast Saccharomyces cerevisiae. Microbiol Mol Biol Rev 62(4):1264–1300
Ernst JF, Pla J (2011) Signaling the glycoshield: maintenance of the Candida albicans cell wall. Int J Med Microbiol 301(5):378–383
Ikner A, Shiozaki K (2005) Yeast signalling pathways in the oxidative stress response. Mutat Res 569(1–2):13–27
Herrero de Dios C, Roman E, Alonso Monge R, Pla J (2010) The role of MAPK signal transduction pathways in the response to oxidative stress in the fungal pathogen Candida albicans: implications in virulence. Curr Protein Pept Sci 11(8):693–703
Saito H, Posas F (2012) Response to hyperosmotic stress. Genetics 192(2):289–318
Navarro-García F, Eisman B, Fiuza SM, Nombela C, Pla J (2005) The MAP kinase Mkc1p is activated under different stress conditions in Candida albicans. Microbiol 151(8):2737–2749
Levin DE (2011) Regulation of cell wall biogenesis in Saccharomyces cerevisiae: the cell wall integrity signaling pathway. Genetics 189(4):1145–1175
Blankenship JR, Fanning S, Hamaker JJ, Mitchell AP (2010) An extensive circuitry for cell wall regulation in Candida albicans. PLoS Pathog 6(2):e1000752
Hirooka T, Ishii H (2013) Chemical control of plant diseases. J Gen Plant Pathol 79(6):390–401
Enjalbert B, Smith DA, Cornell MJ, Alam I, Nicholls S, Brown AJP, Quinn J (2006) Role of Hog-1 stress-activated protein kinase in the global transcriptional response to stress in the fungal pathogen Candida albicans. Mol Biol Cell 17:1018–1032
Alonso-Monge R, Navarro-García F, Román E, Negredo AI, Eisman B, Nombela C, Pla J (2003) The Hog1 mitogen-activated protein kinase is essential in the oxidative stress response and chlamydospore formation in Candida albicans. Eukaryot Cell 2(2):351–361
San José C, Monge RA, Pérez-Díaz R, Pla J, Nombela C (1996) The mitogen-activated protein kinase homolog HOG1 gene controls glycerol accumulation in the pathogenic fungus Candida albicans. J Bacteriol 178(19):5850–5852
Cheetham J, Smith DA, da Silva Dantas A, Doris KS, Patterson MJ, Bruce CR, Quinn J (2007) A single MAPKKK regulates the Hog1 MAPK pathway in the pathogenic fungus Candida albicans. Mol Biol Cell 18(11):4603–4614
Thomas E, Roman E, Claypool S, Manzoor N, Pla J, Panwar SL (2013) Mitochondria influences CDR1 efflux pump activity, Hog1-mediated oxidative stress pathway, iron homeostasis and ergosterol levels in Candida albicans. Antimicrob Agents Chemother 57(11):5580–5599
Montañés FM, Pascual-Ahuir A, Proft M (2011) Repression of ergosterol biosynthesis is essential for stress resistance and is mediated by the Hog1 MAP kinase and the Mot3 and Rox1 transcription factors. Mol Microbiol 79(4):1008–1023
Maeda T, Takekawa M, Saito H (1995) Activation of yeast PBS2 MAPKK by MAPKKKs or by binding of an SH3-containing osmosensor. Science 269(5223):554–558
Maeda T, Wurgler-Murphy S, Saito H (1994) A two-component system that regulates an osmosensing MAP kinase cascade in yeast. Nature 369:242–245
Posas F, Wurgler-Murphy SM, Maeda T, Witten EA, Thai TC, Saito H (1996) Yeast HOG1 MAP kinase cascade is regulated by a multistep phosphorelay mechanism in the SLN1–YPD1–SSK1 “two-component” osmosensor. Cell 86(6):865–875
Jacoby T, Flanagan H, Faykin A, Seto AG, Mattison C, Ota I (1997) Two protein-tyrosine phosphatases inactivate the osmotic stress response pathway in yeast by targeting the mitogen-activated protein kinase, Hog1. J Biol Chem 272(28):17749–17755
Warmka J, Hanneman J, Lee J, Amin D, Ota I (2001) Ptc1, a type 2C Ser/Thr phosphatase, inactivates the HOG pathway by dephosphorylating the mitogen-activated protein kinase Hog1. Mol Cell Biol 21(1):51–60
Arana DM, Nombela C, Alonso-Monge R, Pla J (2005) The Pbs2 MAP kinase kinase is essential for the oxidative-stress response in the fungal pathogen Candida albicans. Microbiol 151(4):1033–1049
Smith DA, Nicholls S, Morgan BA, Brown AJP, Quinn J (2004) A conserved stress-activated protein kinase regulates a core stress response in the human pathogen Candida albicans. Mol Biol Cell 15(9):4179–4190
Ferrigno P, Posas F, Koepp D, Saito H, Silver PA (1998) Regulated nucleo/cytoplasmic exchange of HOG1 MAPK requires the importin [beta] homologs NMD5 and XPO1. EMBO J 17(19):5606–5614
Vr Reiser, Ruis H, Ammerer G (1999) Kinase activity-dependent nuclear export opposes stress-induced nuclear accumulation and retention of Hog1 mitogen-activated protein kinase in the budding yeast Saccharomyces cerevisiae. Mol Biol Cell 10(4):1147–1161
Dixon KP, Jin-Rong X, Smirnoff N, Talbot NJ (1999) Independent signaling pathways regulate cellular turgor during hyperosmotic stress and appressorium-mediated plant infection by Magnaporthe grisea. Plant Cell 11(10):2045–2058
Zhang Y, Lamm R, Pillonel C, Lam S, Xu J-R (2002) Osmoregulation and fungicide resistance: the Neurospora crassa os-2 gene encodes a HOG1 mitogen-activated protein kinase homologue. Appli Environ Microbiol 68(2):532–538
Kojima K, Takano Y, Yoshimi A, Tanaka C, Kikuchi T, Okuno T (2004) Fungicide activity through activation of a fungal signalling pathway. Mol Microbiol 53(6):1785–1796
Segmüller N, Ellendorf U, Tudzynski B, Tudzynski P (2007) BcSAK1, a stress-activated mitogen-activated protein kinase, is involved in vegetative differentiation and pathogenicity in Botrytis cinerea. Eukary Cell 6(2):211–221
Hagiwara D, Asano Y, Marui J, Yoshimi A, Mizuno T, Abe K (2009) Transcriptional profiling for Aspergillus nidulans HogA MAPK signaling pathway in response to fludioxonil and osmotic stress. Fungal Genet Biol 46(11):868–878
Nagygyörgy ED, Hornok L, Ádám AL (2011) Role of MAP kinase signaling in secondary metabolism and adaptation to abiotic/fungicide stress in Fusarium. In: Thajuddin N (ed) Fungicides—beneficial and harmful aspects. InTechWeb, Rijeka, Croatia, pp 167–178
Yoshimi A, Kojima K, Takano Y, Tanaka C (2005) Group III histidine kinase is a positive regulator of Hog1-type mitogen-activated protein kinase in filamentous fungi. Eukaryot Cell 4(11):1820–1828
Zhang X, De Micheli M, Coleman ST, Sanglard D, Moye-Rowley WS (2000) Analysis of the oxidative stress regulation of the Candida albicans transcription factor, Cap1p. Mol Microbiol 36(3):618–629
Alarco A, Raymond M (1999) The bZip transcription factor Cap1p is involved in multidrug resistance and oxidative stress response in Candida albicans. J Bacteriol 181(3):700–708
Temme N, Tudzynski P (2009) Does Botrytis cinerea ignore H2O2-induced oxidative stress during infection? Characterization of Botrytis activator protein 1. Mol Plant Microbe Interact 22(8):987–998
Guo M, Chen Y, Du Y, Dong Y, Guo W, Zhai S, Zhang H, Dong S, Zhang Z, Wang Y, Wang P, Zheng X (2011) The bZIP transcription factor MoAP1 mediates the oxidative stress response and is critical for pathogenicity of the rice blast fungus Magnaporthe oryzae. PLoS Pathog 7(2):e1001302
Lev S, Hadar R, Amedeo P, Baker SE, Yoder OC, Horwitz BA (2005) Activation of an AP1-like transcription factor of the maize pathogen Cochliobolus heterostrophus in response to oxidative stress and plant signals. Eukary Cell 4(2):443–454
Navarro-García F, Sánchez M, Pla J, Nombela C (1995) Functional characterization of the MKC1 gene of Candida albicans, which encodes a mitogen-activated protein kinase homolog related to cell integrity. Mol Cell Biol 15(4):2197–2206
Valiante V, Heinekamp T, Jain R, Härtl A, Brakhage AA (2008) The mitogen-activated protein kinase MpkA of Aspergillus fumigatus regulates cell wall signaling and oxidative stress response. Fungal Genet Biol 45(5):618–627
Kraus PR, Fox DS, Cox GM, Heitman J (2003) The Cryptococcus neoformans MAP kinase Mpk1 regulates cell integrity in response to antifungal drugs and loss of calcineurin function. Mol Microbiol 48(5):1377–1387
Rui O, Hahn M (2007) The Slt2-type MAP kinase Bmp3 of Botrytis cinerea is required for normal saprotrophic growth, conidiation, plant surface sensing and host tissue colonization. Mol Plant Pathol 8(2):173–184
Hou Z, Xue C, Peng Y, Katan T, Kistler HC, Xu J-R (2002) A mitogen-activated protein kinase gene (MGV1) in Fusarium graminearum is required for female fertility, heterokaryon formation, and plant infection. Mol Plant Microbe Interact 15(11):1119–1127
Xu J-R, Staiger CJ, Hamer JE (1998) Inactivation of the mitogen-activated protein kinase Mps1 from the rice blast fungus prevents penetration of host cells but allows activation of plant defense responses. Proc Natl Acad Sci USA 95(21):12713–12718
Levin D (2005) Cell wall integrity signalling in Saccharomyces cerevisiae. Microbiol Mol Biol Rev 69(2):262–291
Lee K, Irie K, Watanabe Y, Araki H, Nishida E, Matsumoto K, Levin D (1993) A yeast mitogen-activated protein kinase homolog (Mpk1p) mediates signalling by protein kinase C. Mol Cell Biol 13(5):3067–3075
Madden K, Sheu Y-J, Baetz K, Andrews B, Snyder M (1997) SBF cell cycle regulator as a target of the yeast PKC-MAP kinase pathway. Science 275(5307):1781–1784
Baetz K, Moffat J, Haynes J, Chang M, Andrews B (2001) Transcriptional coregulation by the cell integrity mitogen-activated protein kinase Slt2 and the cell cycle regulator Swi4. Mol Cell Biol 21(19):6515–6528
Dodou E, Treisman R (1997) The Saccharomyces cerevisiae MADS-box transcription factor Rlm1 is a target for the Mpk1 mitogen-activated protein kinase pathway. Mol Cell Biol 17(4):1848–1859
Jung US, Levin DE (1999) Genome-wide analysis of gene expression regulated by the yeast cell wall integrity signalling pathway. Mol Microbiol 34(5):1049–1057
Garrett-Engele P, Moilanen B, Cyert MS (1995) Calcineurin, the Ca2+/calmodulin-dependent protein phosphatase, is essential in yeast mutants with cell integrity defects and in mutants that lack a functional vacuolar H(+)-ATPase. Mol Cell Biol 15(8):4103–4114
Bonilla M, Cunningham KW (2003) Mitogen-activated protein kinase stimulation of Ca2+ signaling is required for survival of endoplasmic reticulum stress in yeast. Mol Biol Cell 14(10):4296–4305
Zhao C, Jung US, Garrett-Engele P, Roe T, Cyert MS, Levin DE (1998) Temperature-induced expression of yeast FKS2 is under the dual control of protein kinase C and calcineurin. Mol Cell Biol 18(2):1013–1022
Cyert MS (2003) Calcineurin signaling in Saccharomyces cerevisiae: how yeast go crazy in response to stress. Biochem Biophys Res Commun 311(4):1143–1150
Muller EM, Locke EG, Cunningham KW (2001) Differential regulation of two Ca2+ influx systems by pheromone signaling in Saccharomyces cerevisiae. Genetics 159(4):1527–1538
Matheos DP, Kingsbury TJ, Ahsan US, Cunningham KW (1997) Tcn1p/Crz1p, a calcineurin-dependent transcription factor that differentially regulates gene expression in Saccharomyces cerevisiae. Genes Dev 11(24):3445–3458
Stathopoulos-Gerontides A, Guo JJ, Cyert MS (1999) Yeast calcineurin regulates nuclear localization of the Crz1p transcription factor through dephosphorylation. Genes Dev 13(7):798–803
Cunningham KW, Fink GR (1996) Calcineurin inhibits VCX1-dependent H+/Ca2+ exchange and induces Ca2+ ATPases in Saccharomyces cerevisiae. Mol Cell Biol 16(5):2226–2237
Mizunuma M, Hirata D, Miyaoka R, Miyakawa T (2001) GSK-3 kinase Mck1 and calcineurin coordinately mediate Hsl1 down-regulation by Ca2+ in budding yeast. EMBO J 20(5):1074–1085
Imai J, Yahara I (2000) Role of HSP90 in salt stress tolerance via stabilization and regulation of calcineurin. Mol Cell Biol 20(24):9262–9270
Singh SD, Robbins N, Zaas AK, Schell WA, Perfect JR, Cowen LE (2009) Hsp90 governs echinocandin resistance in the pathogenic yeast Candida albicans via calcineurin. PLoS Pathog 5(7):e1000532
Munro CA, Selvaggini S, De Bruijn I, Walker L, Lenardon MD, Gerssen B, Milne S, Brown AJP, Gow NAR (2007) The PKC, HOG and Ca2+ signalling pathways co-ordinately regulate chitin synthesis in Candida albicans. Mol Microbiol 63(5):1399–1413
Choi J, Kim Y, Kim S, Park J, Lee Y-H (2009) MoCRZ1, a gene encoding a calcineurin-responsive transcription factor, regulates fungal growth and pathogenicity of Magnaporthe oryzae. Fungal Genet Biol 46(3):243–254
Ghannoum MA, Rice LB (1999) Antifungal agents: mode of action, mechanisms of resistance, and correlation of these mechanisms with bacterial resistance. Clin Microbiol Rev 12(4):501–517
Denning DW (2003) Echinocandin antifungal drugs. Lancet 362:1142–1151
Baixench M-T, Aoun N, Desnos-Ollivier M, Garcia-Hermoso D, Bretagne S, Ramires S, Piketty C, Dannaoui E (2007) Acquired resistance to echinocandins in Candida albicans: case report and review. J Antimicrob Chemother 59(6):1076–1083
Perlin DS (2011) Current perspectives on echinocandin class drugs. Future Microbiol 6(4):441–457
Walker LA, Gow NAR, Munro CA (2010) Fungal echinocandin resistance. Fungal Genet Biol 47(2):117–126
Bowman SM, Free SJ (2006) The structure and synthesis of the fungal cell wall. BioEssays 28(8):799–808
Reinoso-Martín C, Schüller C, Schuetzer-Muehlbauer M, Kuchler K (2003) The yeast protein kinase C cell integrity pathway mediates tolerance to the antifungal drug caspofungin through activation of Slt2p mitogen-activated protein kinase signaling. Eukaryot Cell 2(6):1200–1210
Miyazaki T, Inamine T, Yamauchi S, Nagayoshi Y, Saijo T, Izumikawa K, Seki M, Kakeya H, Yamamoto Y, Yanagihara K, Miyazaki Y, Kohno S (2010) Role of the Slt2 mitogen-activated protein kinase pathway in cell wall integrity and virulence in Candida glabrata. FEMS Yeast Res 10(3):343–352
Munro CA (2013) Chapter four—chitin and glucan, the yin and yang of the fungal cell wall, implications for antifungal drug discovery and therapy. In: Sima S, Geoffrey MG (eds) Adv Appl Microbiol, vol 83. Academic Press, London, pp 145–172
Walker LA, Munro CA, de Bruijn I, Lenardon MD, McKinnon A, Gow NAR (2008) Stimulation of chitin synthesis rescues Candida albicans from echinocandins. PLoS Pathog 4(4):e1000040
Wiederhold NP, Kontoyiannis DP, Prince RA, Lewis RE (2005) Attenuation of the activity of caspofungin at high concentrations against Candida albicans: possible role of cell wall integrity and calcineurin pathways. Antimicrob Agents Chemother 49(12):5146–5148
Gaughran JP, Lai MH, Kirsch DR, Silverman SJ (1994) Nikkomycin Z is a specific inhibitor of Saccharomyces cerevisiae chitin synthase isozyme Chs3 in vitro and in vivo. J Bacteriol 176(18):5857–5860
Navarro-García F, Alonso-Monge R, Rico H, Pla J, Sentandreu R, Nombela C (1998) A role for the MAP kinase gene MKC1 in cell wall construction and morphological transitions in Candida albicans. Microbiol 144(2):411–424
LaFayette SL, Collins C, Zaas AK, Schell WA, Betancourt-Quiroz M, Gunatilaka AAL, Perfect JR, Cowen LE (2010) PKC signaling regulates drug resistance of the fungal pathogen Candida albicans via circuitry comprised of Mkc1, calcineurin, and Hsp90. PLoS Pathog 6(8):e1001069
Kamada Y, Jung US, Piotrowski J, Levin DE (1995) The protein kinase C-activated MAP kinase pathway of Saccharomyces cerevisiae mediates a novel aspect of the heat shock response. Genes Dev 9(13):1559–1571
Sorgo AG, Heilmann CJ, Dekker HL, Bekker M, Brul S, de Koster CG, de Koning LJ, Klis FM (2011) Effects of fluconazole on the secretome, the wall proteome, and wall integrity of the clinical fungus Candida albicans. Eukaryot Cell 10(8):1071–1081
Jandrositz A, Turnowsky F, Högenauer G (1991) The gene encoding squalene epoxidase from Saccharomyces cerevisiae: cloning and characterization. Gene 107(1):155–160
Marcireau C, Guilloton M, Karst F (1990) In vivo effects of fenpropimorph on the yeast Saccharomyces cerevisiae and determination of the molecular basis of the antifungal property. Antimicrob Agents Chemother 34(6):989–993
Hartsel S, Bolard J (1996) Amphotericin B: new life for an old drug. Trends Pharmacol Sci 17(12):445–449
Kelly J, Rowan R, McCann M, Kavanagh K (2009) Exposure to caspofungin activates Cap and Hog pathways in Candida albicans. Med Mycol 47(7):697–706
Alonso-Monge R, Navarro-García F, Molero G, Diez-Orejas R, Gustin M, Pla J, Sánchez M, Nombela C (1999) Role of the mitogen-activated protein kinase Hog1p in morphogenesis and virulence of Candida albicans. J Bacteriol 181(10):3058–3068
Torres-Quiroz F, García-Marqués S, Coria R, Randez-Gil F, Prieto JA (2010) The activity of yeast Hog1 MAPK Is required during endoplasmic reticulum stress induced by tunicamycin exposure. J Biol Chem 285(26):20088–20096
Gullino ML, Leroux P, Smith CM (2000) Uses and challenges of novel compounds for plant disease control. Crop Protect 19(1):1–11
Pillonel C, Meyer T (1997) Effect of phenyl pyrroles on glycerol accumulation and protein kinase activity of Neurospora crassa. Pestic Sci 49:229–236
Yaakov G, Bell M, Hohmann S, Engelberg D (2003) Combination of two activating mutations in one HOG1 gene forms hyperactive enzymes that induce growth arrest. Mol Cell Biol 23(14):4826–4840
Motoyama T, Ochiai N, Morita M, Iida Y, Usami R, Kudo T (2008) Involvement of putative response regulator genes of the rice blast fungus Magnaporthe oryzae in osmotic stress response, fungicide action, and pathogenicity. Curr Genet 54(4):185–195
Bahn Y-S, Kojima K, Cox GM, Heitman J (2005) Specialization of the HOG pathway and its impact on differentiation and virulence of Cryptococcus neoformans. Mol Biol Cell 16(5):2285–2300
Kojima K, Bahn Y-S, Heitman J (2006) Calcineurin, Mpk1 and Hog1 MAPK pathways independently control fludioxonil antifungal sensitivity in Cryptococcus neoformans. Microbiol 152(3):591–604
Ochiai N, Fujimura M, Oshima M, Motoyama T, Ichiishi A, Yamada-Okabe H, Yamaguchi I (2002) Effects of iprodione and fludioxonil on glycerol synthesis and hyphal development in Candida albicans. Biosci Biotech Biochem 66(10):2209–2215
Merchan S, Bernal D, Serrano R, Yenush L (2004) Response of the Saccharomyces cerevisiae Mpk1 mitogen-activated protein kinase pathway to increases in internal turgor pressure caused by loss of Ppz protein phosphatases. Eukaryot Cell 3(1):100–107
Alarco A-M, Balan I, Talibi D, Mainville N, Raymond M (1997) AP1-mediated multidrug resistance in Saccharomyces cerevisiae requires FLR1 encoding a transporter of the major facilitator superfamily. J Biol Chem 272(31):19304–19313
Schubert S, Barker KS, Znaidi S, Schneider S, Dierolf F, Dunkel N, Aïd M, Boucher G, Rogers PD, Raymond M, Morschhäuser J (2011) Regulation of efflux pump expression and drug resistance by the transcription factors Mrr1, Upc2, and Cap1 in Candida albicans. Antimicrob Agents Chemother 55(5):2212–2223
Ko Y-J, Yu YM, Kim G-B, Lee G-W, Maeng PJ, Kim S, Floyd A, Heitman J, Bahn Y-S (2009) Remodeling of global transcription patterns of Cryptococcus neoformans genes mediated by the stress-activated HOG signaling pathways. Eukaryot Cell 8(8):1197–1217
Thevissen K, de Mello Tavares P, Xu D, Blankenship J, Vandenbosch D, Idkowiak-Baldys J, Govaert G, Bink A, Rozental S, de Groot PW, Davis TR, Kumamoto CA, Vargas G, Nimrichter L, Coenye T, Mitchell A, Roemer T, Hannun YA, Cammue BP (2012) The plant defensin RsAFP2 induces cell wall stress, septin mislocalization and accumulation of ceramides in Candida albicans. Mol Microbiol 84(1):166–180
Thevissen K, Warnecke DC, Francois IEJA, Leipelt M, Heinz E, Ott C, Zahringer U, Thomma BPHJ, Ferket KKA, Cammue BPA (2004) Defensins from insects and plants interact with fungal glucosylceramides. J Biol Chem 279(6):3900–3905
Koo JC, Lee B, Young ME, Koo SC, Cooper JA, Baek D, Lim CO, Lee SY, Yun DJ, Cho MJ (2004) Pn-AMP1, a plant defense protein, induces actin depolarization in yeasts. Plant Cell Physiol 45(11):1669–1680
Hayes BME, Bleackley MR, Wiltshire JL, Anderson MA, Traven A, van der Weerden NL (2013) Identification and mechanism of action of the plant defensin NaD1 as a new member of the antifungal drug arsenal against Candida albicans. Antimicrob Agents Chemother 57(8):3667–3675
Ramamoorthy V, Zhao X, Snyder AK, Xu J-R, Shah DM (2007) Two mitogen-activated protein kinase signalling cascades mediate basal resistance to antifungal plant defensins in Fusarium graminearum. Cell Microbiol 9(6):1491–1506
Jenczmionka N, Maier F, Lösch A, Schäfer W (2003) Mating, conidiation and pathogenicity of Fusarium graminearum, the main causal agent of the head-blight disease of wheat, are regulated by the MAP kinase gpmk1. Curr Genet 43(2):87–95
Hagen S, Marx F, Ram AF, Meyer V (2007) The antifungal protein AFP from Aspergillus giganteus inhibits chitin synthesis in sensitive fungi. Appl Environ Microbiol 73(7):2128–2134
Ouedraogo JP, Hagen S, Spielvogel A, Engelhardt S, Meyer V (2011) Survival strategies of yeast and filamentous fungi against the antifungal protein AFP. J Biol Chem 286(16):13859–13868
Vylkova S, Jang WS, Li W, Nayyar N, Edgerton M (2007) Histatin 5 initiates osmotic stress response in Candida albicans via activation of the Hog1 mitogen-activated protein kinase pathway. Eukaryot Cell 6(10):1876–1888
Argimon S, Fanning S, Blankenship JR, Mitchell AP (2011) Interaction between the Candida albicans high-osmolarity glycerol (HOG) pathway and the response to human b-Defensins 2 and 3. Eukaryot Cell 10(2):272–275
van der Weerden NL, Lay FT, Anderson MA (2008) The plant defensin, NaD1, enters the cytoplasm of Fusarium oxysporum hyphae. J Biol Chem 283(21):14445–14452
Aerts AM, Bammens L, Govaert G, Carmona-Gutierrez D, Madeo F, Cammue BP, Thevissen K (2011) The antifungal plant defensin HsAFP1 from Heuchera sanguinea induces apoptosis in Candida albicans. Front Microbiol 2:1–9
Sussman A, Huss K, Chio L-C, Heidler S, Shaw M, Ma D, Zhu G, Campbell RM, Park T-S, Kulanthaivel P, Scott JE, Carpenter JW, Strege MA, Belvo MD, Swartling JR, Fischl A, Yeh W-K, Shih C, Ye XS (2004) Discovery of cercosporamide, a known antifungal natural product, as a selective Pkc1 kinase inhibitor through high-throughput screening. Eukaryot Cell 3(4):932–943
Del Poeta M, Cruz MC, Cardenas ME, Perfect JR, Heitman J (2000) Synergistic antifungal activities of bafilomycin A1, fluconazole, and the pneumocandin MK-0991/caspofungin acetate (L-743,873) with calcineurin inhibitors FK506 and L-685,818 against Cryptococcus neoformans. Antimicrob Agents Chemother 44(3):739–746
Onyewu C, Blankenship JR, Del Poeta M, Heitman J (2003) Ergosterol biosynthesis inhibitors become fungicidal when combined with calcineurin inhibitors against Candida albicans, Candida glabrata, and Candida krusei. Antimicrob Agents Chemother 47(3):956–964
McClean MN, Mody A, Broach JR, Ramanathan S (2007) Cross-talk and decision making in MAP kinase pathways. Nat Genet 39(3):409–414
Juvvadi PR, Gehrke C, Fortwendel JR, Lamoth F, Soderblom EJ, Cook EC, Hast MA, Asfaw YG, Moseley MA, Creamer TP, Steinbach WJ (2013) Phosphorylation of calcineurin at a novel serine-proline rich region orchestrates hyphal growth and virulence in Aspergillus fumigatus. PLoS Pathog 9(8):e1003564
Acknowledgments
This work was supported by a Discovery Project from the Australian Research Council (ARC, DP120102694). B. H. was supported by an Australian Postgraduate Award. Work in the A.T. lab on C. albicans is funded by the Australian National Health and Medical Research Council (NHMRC), and the Monash University Researcher Accelerator (MRA) Grant.
Author information
Authors and Affiliations
Corresponding authors
Additional information
N. L. van der Weerden and M. R. Bleackley contributed equally.
Rights and permissions
About this article
Cite this article
Hayes, B.M.E., Anderson, M.A., Traven, A. et al. Activation of stress signalling pathways enhances tolerance of fungi to chemical fungicides and antifungal proteins. Cell. Mol. Life Sci. 71, 2651–2666 (2014). https://doi.org/10.1007/s00018-014-1573-8
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00018-014-1573-8