Cellular and Molecular Life Sciences

, Volume 71, Issue 9, pp 1623–1639

In vitro neurogenesis: development and functional implications of iPSC technology

Authors

    • Unit of Neuromuscular and Neurodegenerative Disorders, Laboratory of Molecular Medicine, Department of NeurosciencesBambino Gesù Children’s Research Hospital, IRCCS
  • Monica Nizzardo
    • Department of Neurological Sciences, Dino Ferrari Centre, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Foundation, IRCCS Foundation Ca’GrandaUniversity of Milan
  • Stefania Corti
    • Department of Neurological Sciences, Dino Ferrari Centre, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Foundation, IRCCS Foundation Ca’GrandaUniversity of Milan
  • Ginevra Zanni
    • Unit of Neuromuscular and Neurodegenerative Disorders, Laboratory of Molecular Medicine, Department of NeurosciencesBambino Gesù Children’s Research Hospital, IRCCS
  • Enrico Bertini
    • Unit of Neuromuscular and Neurodegenerative Disorders, Laboratory of Molecular Medicine, Department of NeurosciencesBambino Gesù Children’s Research Hospital, IRCCS
Review

DOI: 10.1007/s00018-013-1511-1

Cite this article as:
Compagnucci, C., Nizzardo, M., Corti, S. et al. Cell. Mol. Life Sci. (2014) 71: 1623. doi:10.1007/s00018-013-1511-1

Abstract

Neurogenesis is the developmental process regulating cell proliferation of neural stem cells, determining their differentiation into glial and neuronal cells, and orchestrating their organization into finely regulated functional networks. Can this complex process be recapitulated in vitro using induced pluripotent stem cell (iPSC) technology? Can neurodevelopmental and neurodegenerative diseases be modeled using iPSCs? What is the potential of iPSC technology in neurobiology? What are the recent advances in the field of neurological diseases? Since the applications of iPSCs in neurobiology are based on the capacity to regulate in vitro differentiation of human iPSCs into different neuronal subtypes and glial cells, and the possibility of obtaining iPSC-derived neurons and glial cells is based on and hindered by our poor understanding of human embryonic development, we reviewed current knowledge on in vitro neural differentiation from a developmental and cellular biology perspective. We highlight the importance to further advance our understanding on the mechanisms controlling in vivo neurogenesis in order to efficiently guide neurogenesis in vitro for cell modeling and therapeutical applications of iPSCs technology.

Keywords

iPSCsNeurogenesisNeurological disease modeling

Copyright information

© Springer Basel 2013