Cellular and Molecular Life Sciences

, Volume 68, Issue 17, pp 2907–2917

The FN3 and BRCT motifs in the exomer component Chs5p define a conserved module that is necessary and sufficient for its function

  • Rebeca Martín-García
  • Nagore de León
  • Mohammad Reza Sharifmoghadam
  • M.-Ángeles Curto
  • Marta Hoya
  • Pilar Bustos-Sanmamed
  • M.-Henar Valdivieso
Research Article

DOI: 10.1007/s00018-010-0596-z

Cite this article as:
Martín-García, R., de León, N., Sharifmoghadam, M.R. et al. Cell. Mol. Life Sci. (2011) 68: 2907. doi:10.1007/s00018-010-0596-z

Abstract

Chs5p is a component of the exomer, a coat complex required to transport the chitin synthase Chs3p from the trans-Golgi network to the plasma membrane. The Chs5p N-terminal region exhibits fibronectin type III (FN3) and BRCT domains. FN3 domains are present in proteins that mediate adhesion processes, whereas BRCT domains are involved in DNA repair. Several fungi—including Schizosaccharomyces pombe, which has no detectable amounts of chitin—have proteins similar to Chs5p. Here we show that the FN3 and BRCT motifs in Chs5p behave as a module that is necessary and sufficient for Chs5p localization and for cargo delivery. The N-terminal regions of S. cerevisiae Chs5p and S. pombe Cfr1p are interchangeable in terms of Golgi localization, but not in terms of exomer assembly, showing that the conserved function of this module is protein retention in this organelle and that the interaction between the exomer components is organism-specific.

Keywords

Yeast Cell wall Chitin Golgi Vesicular trafficking 

Abbreviations

TGN

Trans-Golgi network

FN3

Fibronectin type III

BRCT

Breast cancer susceptibility protein C-terminal domain

ChAps

Chs5p-Arf1p-binding proteins

GFP

Green fluorescent protein

RFP

Red fluorescent protein

Supplementary material

18_2010_596_MOESM1_ESM.tif (9.1 mb)
Supplementary material 1 (TIFF 9,274 kb)
18_2010_596_MOESM2_ESM.tif (3.2 mb)
Supplementary material 2 (TIFF 3,236 kb)

Copyright information

© Springer Basel AG 2010

Authors and Affiliations

  • Rebeca Martín-García
    • 1
  • Nagore de León
    • 1
  • Mohammad Reza Sharifmoghadam
    • 1
    • 2
  • M.-Ángeles Curto
    • 1
  • Marta Hoya
    • 1
  • Pilar Bustos-Sanmamed
    • 1
  • M.-Henar Valdivieso
    • 1
  1. 1.Departamento de Microbiología y Genética/Instituto de Microbiología BioquímicaUniversidad de Salamanca/CSICSalamancaSpain
  2. 2.Faculty of Veterinary MedicineZabol UniversityZabolIran

Personalised recommendations