Cellular and Molecular Life Sciences

, Volume 68, Issue 12, pp 2141–2156

Mesenchymal stem cells or cardiac progenitors for cardiac repair? A comparative study

  • Remco Koninckx
  • Annick Daniëls
  • Severina Windmolders
  • Françoise Carlotti
  • Urbain Mees
  • Paul Steels
  • Jean-Luc Rummens
  • Marc Hendrikx
  • Karen Hensen
Research Article

DOI: 10.1007/s00018-010-0560-y

Cite this article as:
Koninckx, R., Daniëls, A., Windmolders, S. et al. Cell. Mol. Life Sci. (2011) 68: 2141. doi:10.1007/s00018-010-0560-y

Abstract

In the past, clinical trials transplanting bone marrow–derived mononuclear cells reported a limited improvement in cardiac function. Therefore, the search for stem cells leading to more successful stem cell therapies continues. Good candidates are the so-called cardiac stem cells (CSCs). To date, there is no clear evidence to show if these cells are intrinsic stem cells from the heart or mobilized cells from bone marrow. In this study we performed a comparative study between human mesenchymal stem cells (hMSCs), purified c-kit+ CSCs, and cardiosphere-derived cells (CDCs). Our results showed that hMSCs can be discriminated from CSCs by their differentiation capacity towards adipocytes and osteocytes and the expression of CD140b. On the other hand, cardiac progenitors display a greater cardiomyogenic differentiation capacity. Despite a different isolation protocol, no distinction could be made between c-kit+ CSCs and CDCs, indicating that they probably derive from the same precursor or even are the same cells.

Keywords

Mesenchymal stem cellsCardiosphere-derived cellsc-kit+ cardiac stem cellsHeart failureMyocardial infarctionDifferentiationCoculture

Copyright information

© Springer Basel AG 2010

Authors and Affiliations

  • Remco Koninckx
    • 1
    • 2
  • Annick Daniëls
    • 1
  • Severina Windmolders
    • 1
    • 2
  • Françoise Carlotti
    • 3
  • Urbain Mees
    • 4
  • Paul Steels
    • 2
  • Jean-Luc Rummens
    • 1
    • 2
  • Marc Hendrikx
    • 2
    • 4
  • Karen Hensen
    • 1
  1. 1.Laboratory of Experimental HematologyJessa HospitalHasseltBelgium
  2. 2.Laboratory of Physiology, Faculty of Medicine, and School of Life SciencesHasselt University, Biomedical Research Institute and Transnational University LimburgDiepenbeekBelgium
  3. 3.Department of Molecular Cell BiologyLeiden University Medical CenterLeidenThe Netherlands
  4. 4.Department of Cardiothoracic SurgeryJessa HospitalHasseltBelgium