Cellular and Molecular Life Sciences

, Volume 67, Issue 22, pp 3763–3784

Genome-wide analysis of plant metal transporters, with an emphasis on poplar

  • Aude Migeon
  • Damien Blaudez
  • Olivia Wilkins
  • Barbara Montanini
  • Malcolm M. Campbell
  • Pierre Richaud
  • Sébastien Thomine
  • Michel Chalot
Review

DOI: 10.1007/s00018-010-0445-0

Cite this article as:
Migeon, A., Blaudez, D., Wilkins, O. et al. Cell. Mol. Life Sci. (2010) 67: 3763. doi:10.1007/s00018-010-0445-0

Abstract

The specific transport of metal ions, mediated by membrane-localized metal transporters, is of fundamental importance in all eukaryotes. Genome-wide analysis of metal transporters was undertaken, making use of whole genome sequences of the green alga Chlamydomonas reinhardtii, the moss Physcomitrella patens, the lycophyte Selaginella moellendorffii, the monocots rice and sorghum, and the dicots Arabidopsis thaliana, poplar, grapevine, as well as of the yeast Saccharomyces cerevisiae. A repertoire of 430 metal transporters was found in total across eight photosynthetic plants, as well as in S. cerevisiae. Seventy-two full-length metal transporter genes were identified in the Populus genome alone, which is the largest number of metal transporters genes identified in any single species to date. Diversification of some transporter family gene clusters appears to have occurred in a lineage-specific manner. Expression analysis of Populus metal transporters indicates that some family members show tissue-specific transcript abundance. Taken together, the data provide a picture into the diversification of these important gene families.

Keywords

Expression evidenceGene diversificationMetal transportersPopulus trichocarpa

Supplementary material

18_2010_445_MOESM1_ESM.doc (225 kb)
Supplementary material 1 (DOC 225 kb)
18_2010_445_MOESM2_ESM.doc (788 kb)
Supplementary material 2 (DOC 787 kb)
18_2010_445_MOESM3_ESM.ppt (560 kb)
Figure S1. An unrooted, neighbor-joining (NJ)-based tree of the zinc–iron permeases (ZIP) family in selected plants. The analysis was performed as described in the “Materials and methods” section and the tree was generated using MEGA version 4.1 [28] after sequence alignment. Distances were calculated with the Poisson correction model for distance computation (homogeneous substitution pattern among lineages). Bootstrap values are indicated (1,000 replicates). Branch lengths are proportional to phylogenetic distances. Corresponding gene loci are given in a supplemental file. Names of the species are given in the legend of Fig. 1. Supplementary material 3 (PPT 559 kb)

Copyright information

© Springer Basel AG 2010

Authors and Affiliations

  • Aude Migeon
    • 1
  • Damien Blaudez
    • 1
  • Olivia Wilkins
    • 2
  • Barbara Montanini
    • 3
  • Malcolm M. Campbell
    • 2
  • Pierre Richaud
    • 4
    • 5
    • 6
  • Sébastien Thomine
    • 7
  • Michel Chalot
    • 1
  1. 1.UMR INRA/UHP 1136 “Tree–microbe Interactions”, Faculty of Sciences and TechnologyNancy-UniversityVandoeuvre-les-NancyFrance
  2. 2.Department of Cell and Systems Biology and Centre for the Analysis of Genome Evolution and FunctionUniversity of TorontoTorontoCanada
  3. 3.Dipartimento di Biochimica e Biologia MolecolareUniversità degli Studi di ParmaParmaItaly
  4. 4.Laboratoire des Echanges Membranaires et SignalisationCEA, DSV, iBEBSt. Paul les DuranceFrance
  5. 5.CNRS, UMR 6191St. Paul les DuranceFrance
  6. 6.Université Aix-MarseilleSt. Paul les DuranceFrance
  7. 7.Institut des Sciences du Végétal, CNRSGif-sur-YvetteFrance