Review

Cellular and Molecular Life Sciences

, Volume 67, Issue 16, pp 2717-2747

First online:

Retroviral reverse transcriptases

  • Alon HerschhornAffiliated withDepartment of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University
  • , Amnon HiziAffiliated withDepartment of Cell and Developmental Biology, Sackler School of Medicine, Tel Aviv University Email author 

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Reverse transcription is a critical step in the life cycle of all retroviruses and related retrotransposons. This complex process is performed exclusively by the retroviral reverse transcriptase (RT) enzyme that converts the viral single-stranded RNA into integration-competent double-stranded DNA. Although all RTs have similar catalytic activities, they significantly differ in several aspects of their catalytic properties, their structures and subunit composition. The RT of human immunodeficiency virus type-1 (HIV-1), the virus causing acquired immunodeficiency syndrome (AIDS), is a prime target for the development of antiretroviral drug therapy of HIV-1/AIDS carriers. Therefore, despite the fundamental contributions of other RTs to the understanding of RTs and retrovirology, most recent RT studies are related to HIV-1 RT. In this review we summarize the basic properties of different RTs. These include, among other topics, their structures, enzymatic activities, interactions with both viral and host proteins, RT inhibition and resistance to antiretroviral drugs.

Keywords

Reverse transcription Retroviruses DNA and RNA-dependent DNA synthesis Ribonuclease H RT inhibitors HIV Drug resistance