Cellular and Molecular Life Sciences

, Volume 67, Issue 11, pp 1831–1843

Thyroid hormone receptor-mediated regulation of the methionine adenosyltransferase 1 gene is associated with cell invasion in hepatoma cell lines

  • Sheng-Ming Wu
  • Ya-Hui Huang
  • Yi-Hsin Lu
  • Ling-Fang Chien
  • Chau-Ting Yeh
  • Ming-Ming Tsai
  • Chen-Hsin Liao
  • Wei-Jan Chen
  • Chia-Jung Liao
  • Wan-Li Cheng
  • Kwang-Huei Lin
Research Article

DOI: 10.1007/s00018-010-0281-2

Cite this article as:
Wu, SM., Huang, YH., Lu, YH. et al. Cell. Mol. Life Sci. (2010) 67: 1831. doi:10.1007/s00018-010-0281-2

Abstract

The thyroid hormone T3 regulates differentiation, growth, and development. We demonstrated that methionine adenosyltransferase 1A (MAT1A) was positively regulated by T3 identified by cDNA microarray previously. The expression of the MAT1A was upregulated by T3 in hepatoma cell lines overexpressing thyroid hormone receptors (TRs). Additionally, these findings indicate that MAT1A may be regulated by CCAAT/enhancer binding protein (C/EBP). The critical role of the C/EBP binding sites was confirmed by the reporter or chromatin immuno-precipitation (ChIP) assay. In addition, C/EBP was upregulated in hepatoma cells after T3 treatment and ectopic expression of MAT1A inhibited cell migration and invasion in J7 hepatoma cells. Conversely, knockdown of MAT1A expression increased cell migration. Together, these findings suggest that the expression of the MAT1A gene is mediated by C/EBP and is indirectly upregulated by T3. Finally, TR was downregulated in a small subset of hepatocellular carcinoma cells concomitantly reduced the expression of C/EBPα and MAT1A.

Keywords

MAT1A Thyroid hormone Receptor Invasion Migration 

Copyright information

© Springer Basel AG 2010

Authors and Affiliations

  • Sheng-Ming Wu
    • 1
  • Ya-Hui Huang
    • 2
  • Yi-Hsin Lu
    • 1
  • Ling-Fang Chien
    • 1
  • Chau-Ting Yeh
    • 3
    • 4
  • Ming-Ming Tsai
    • 1
    • 5
  • Chen-Hsin Liao
    • 1
  • Wei-Jan Chen
    • 6
  • Chia-Jung Liao
    • 1
  • Wan-Li Cheng
    • 1
  • Kwang-Huei Lin
    • 1
  1. 1.Department of Biochemistry, School of MedicineChang-Gung UniversityTaoyuanTaiwan, ROC
  2. 2.Medical Research Center, Chang-Gung Memorial HospitalTaoyuanTaiwan, ROC
  3. 3.Department of Medicine, College of MedicineChang-Gung UniversityTaoyuanTaiwan, ROC
  4. 4.Liver Research Unit, Chang-Gung Medical CenterTaipeiTaiwan, ROC
  5. 5.Department of NursingChang-Gung Institute of TechnologyTaoyuanTaiwan, ROC
  6. 6.First Cardiovascular DivisionChang Gung Memorial HospitalTaoyuanTaiwan, ROC

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